Nucleotide sequence and exact localization of the neomycin phosphotransferase gene from transposon Tn5.

Beck E; Ludwig G; Auerswald EA; Reiss B; Schaller H

doi:10.1016/0378-1119(82)90023-3

Abstract

The nucleotide sequence of 1200 bp from the unique region of transposon Tn5 containing the neomycin phosphotransferase gene (neo) was determined, and the location of the neo gene was identified by deletion mutants in a translational reading frame of 792 bp. The derived gene product, an aminoglycoside 3'-phosphotransferase (APH) II, consists of 264 amino acid residues and has a calculated Mr of 29053. Its amino acid sequence shows sequence homologies to the APH type I enzyme coded for by transposon Tn903 (Oka et al., 1981).

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References

Articles referenced by this article (27)

Structural analysis of Tn5
Auerswald
1981
Construction of plasmids carrying the cI gene of bacteriophage A
Backman
1976
Nucleotide sequence of the gene ompA coding the outer membrane protein II of Escherichia coli K-12.
Beck E, Bremer E
Nucleic Acids Res, (13):3011-3027 1980
MED: 6253901
Transposition of R-factor genes to bacteriophage λ
Berg
1975
Insertion, excision, and inversion of Tn 5
Berg
1981
Export of a protein into the outer membrane of Escherichia coli K12. Stable incorporation of the OmpA protein requires less than 193 amino-terminal amino-acid residues.
Bremer E, Cole ST, Hindennach I, Henning U, Beck E, Kurz C, Schaller H
Eur J Biochem, (1):223-231 1982
MED: 7037401
Transposition protein of Tn3: identification and characterisation of an essential repressor-controlled gene product.
Chou J, Lemaux PG, Casadaban MJ, Cohen SN
Nature, (5741):801-806 1979
MED: 390402
A new dominant hybrid selective marker for higher eukaryotic cells.
Colbere-Garapin F, Horodniceanu F, Kourilsky P, Garapin AC
J Mol Biol, (1):1-14 1981
MED: 6271971
Improvements of DNA sequencing gels.
Garoff H, Ansorge W
Anal Biochem, (2):450-457 1981
MED: 7304971
Transposition of a DNA sequence determining kanamycin resistance into the single-stranded genome of bacteriophage fd.
Herrmann R, Neugebauer K, Zentgraf H, Schaller H
Mol Gen Genet, (2):171-178 1978
MED: 345091

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Data

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This data has been provided by curated databases and other sources that have cited the article.

Proteins in UniProt (4)

Transposon Tn5 left end with reading frame of unknown protein(UniProt - Q57414)
Bleomycin resistance protein(UniProt - Q99399)
Transposase IS4-like domain-containing protein(UniProt - Q47660)
Aminoglycoside 3'-phosphotransferase(UniProt - P00552)

Nucleotide sequences in ENA (Showing 5 of 15)

Escherichia coli neomycin phosphotransferase(ENA - AAA85506)
Shuttle cosmid vector pFD666 aminoglycoside phosphotransferase(ENA - AAB60865)
Escherichia coli hypothetical protein(ENA - AAA73388)
Shuttle cosmid vector pFD666, complete sequence.(ENA - AF000429)
Escherichia coli hypothetical protein(ENA - AAA73389)

Go to all (15) records in ENA

Search life-sciences literature (45,103,589 articles, preprints and more)

Nucleotide sequence and exact localization of the neomycin phosphotransferase gene from transposon Tn5.

Abstract

Full text links

References

Structural analysis of Tn5

Construction of plasmids carrying the cI gene of bacteriophage A

Nucleotide sequence of the gene ompA coding the outer membrane protein II of Escherichia coli K-12.

Transposition of R-factor genes to bacteriophage λ

Insertion, excision, and inversion of Tn 5

Export of a protein into the outer membrane of Escherichia coli K12. Stable incorporation of the OmpA protein requires less than 193 amino-terminal amino-acid residues.

Transposition protein of Tn3: identification and characterisation of an essential repressor-controlled gene product.

A new dominant hybrid selective marker for higher eukaryotic cells.

Improvements of DNA sequencing gels.

Transposition of a DNA sequence determining kanamycin resistance into the single-stranded genome of bacteriophage fd.

Citations & impact

Impact metrics

Citations of article over time

Alternative metrics

Smart citations by scite.ai
Explore citation contexts and check if this article has been supported or disputed.
https://scite.ai/reports/10.1016/0378-1119(82)90023-3

Article citations

Protocol for the application of single-cell damage in murine intestinal organoid models.

Genetically engineered eucalyptus expressing pesticidal proteins from <i>Bacillus thuringiensis</i> for insect resistance: a risk assessment evaluation perspective.

Safety Assessment of the CP4 EPSPS and NPTII Proteins in Eucalyptus.

Segregational drift hinders the evolution of antibiotic resistance on polyploid replicons.

Sulfadiazine and phosphinothricin selection systems optimised for the transformation of tobacco BY-2 cells.

Data

Data that cites the article

Proteins in UniProt (4)

Nucleotide sequences in ENA (Showing 5 of 15)

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