Two distinct pathways of specific killing revealed by perforin mutant cytotoxic T lymphocytes.

Kojima H; Shinohara N; Hanaoka S; Someya-Shirota Y; Takagaki Y; Ohno H; Saito T; Katayama T; Yagita H; Okumura K

doi:10.1016/1074-7613(94)90066-3

Two distinct pathways of specific killing revealed by perforin mutant cytotoxic T lymphocytes.

Ohno H ,

Affiliations

1. Laboratory of Cellular Immunology, Mitsubishi Kasei Institute of Life Sciences, Tokyo, Japan.
Authors
Kojima H¹
(1 author)

ORCIDs linked to this article

Ohno H | 0000-0001-8776-9661

Immunity, 01 Aug 1994, 1(5):357-364
https://doi.org/10.1016/1074-7613(94)90066-3 PMID: 7533644

Abstract

To study the contribution of putative perforin-independent mechanism in the antigen-specific target destruction by cytotoxic T lymphocytes CD8+ CTL lines were established from spleen cells of chimeric mice produced by injecting perforin (-/-) embryonic stem cells into blastocysts of RAG-2(-/-) mice. When tested on normal concanavalin A blasts, these perforin-deficient cytotoxic T lymphocyte lines were found to be capable of inducing antigen-specific target cell lysis accompanied by DNA degradation. In contrast, with target cells carrying a mutation in Fas molecule, perforin-independent cytotoxicity was not detectable. These data not only confirmed the primary role of perforin but simultaneously revealed a major contribution of a perforin-independent Fas-mediated pathway in antigen-specific cytolysis.

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V(D)J recombination-activating protein 2(UniProt - B7ZMX3)
V(D)J recombination-activating protein 2(UniProt - Q8CD87)
V(D)J recombination-activating protein 2(UniProt - P21784)
V(D)J recombination-activating protein 2(UniProt - Q542D1)
Perforin 1 (Pore forming protein)(UniProt - A2RSY7)

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NIAID NIH HHS (1)

Grant ID: AI20047
63 publications

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Two distinct pathways of specific killing revealed by perforin mutant cytotoxic T lymphocytes.

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Abstract

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References

Functions and mechanisms of lysis induced by cytotoxic T lymphocytes and natural killer cells

RAG-2-deficient blastocyst complementation: an assay of gene function in lymphocyte development

Extracellular ATP in T-lymphocyte activation: possible role in effector functions

Fas and its ligand in general mechanism of T cell-mediated cytotoxicity

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Mouse Genome Informatics (MGI)

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