Europe PMC

This website requires cookies, and the limited processing of your personal data in order to function. By using the site you are agreeing to this as outlined in our privacy notice and cookie policy.

In vitro lipolytic effect of leptin on mouse adipocytes: evidence for a possible autocrine/paracrine role of leptin.

Author information

Affiliations

  • 1. Department of Physiology and Nutrition, University of Navarra, Pamplona, Spain.
      Authors
    • Frühbeck G 1
    (1 author)

ORCIDs linked to this article

Biochemical and Biophysical Research Communications, 01 Nov 1997, 240(3):590-594
https://doi.org/10.1006/bbrc.1997.7716 PMID: 9398609 

Abstract 

The present study has examined the effects of the adipocyte-derived hormone, leptin, on lipolysis in fat cells of different types of mice. Exposure to leptin (1.25.10(-6) M to 1.25.10(-12) M) increased (P < 0.01) the lipolytic activity of fat cells obtained from lean mice. A greater stimulation was observed when adipocytes from ob/ob mice were examined. Throughout the concentrations tested, the leptin-induced lipolysis observed in fat cells of lean animals was smaller than that obtained in ob/ob mice. The maximal lipolytic effect in obese animals was observed with 10(-8) M of OB protein. The lipolytic activity following the addition of 1.25.10(-10) M to 1.25.10(-6) M was significantly increased (P < 0.01) in ob/ob mice compared to lean animals. Adipocytes from ob/ob mice responded in a dose-dependent manner to the OB protein, while the leptin-induced lipolysis observed in lean animals was dose-independent. In contrast to lean and ob/ob mice, leptin did not stimulate lipolysis in adipocytes from db/db mice, which have a mutation in the leptin receptor gene. These in vitro studies suggest an autocrine/paracrine action of leptin on white fat cells and envisages the involvement of the OB protein, not only in centrally mediated pathways, but also in physiological functions which take place peripherally.

Full text links 

Read article at publisher's site: https://doi.org/10.1006/bbrc.1997.7716

References 

Articles referenced by this article (30)

Show 10 more references (10 of 30)

Citations & impact 

Impact metrics

149
Citations
Jump to Citations
6
Data citations
Jump to Data

Citations of article over time

Article citations

Go to all (149) article citations

Data 

Similar Articles 

To arrive at the top five similar articles we use a word-weighted algorithm to compare words from the Title and Abstract of each citation.