Use of green fluorescent protein to detect expression of nif genes of Azoarcus sp. BH72, a grass-associated diazotroph, on rice roots.

Egener T; Hurek T; Reinhold-Hurek B

doi:10.1094/mpmi.1998.11.1.71

Use of green fluorescent protein to detect expression of nif genes of Azoarcus sp. BH72, a grass-associated diazotroph, on rice roots.

Egener T ¹,

Hurek T ,

Reinhold-Hurek B

Affiliations

1. Max-Planck-Institut für terrestrische Mikrobiologie, Arbeitsgruppe Symbioseforschung, Marburg, Germany.
Authors
Egener T¹
(1 author)

Molecular Plant-microbe Interactions : MPMI, 01 Jan 1998, 11(1):71-75
https://doi.org/10.1094/mpmi.1998.11.1.71 PMID: 9425688

Abstract

A gfp (green fluorescent protein) cassette for transcriptional fusions has been developed to study gene expression in Azoarcus sp. BH72 in association with plant roots. The bacteria expressed nitrogenase genes (nifHDK) in the rhizosphere, on root tips, and in epidermal cells of rice seedlings. Green fluorescent protein fusions also visualized promoter activity of single cells in soil.

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Read article at publisher's site: https://doi.org/10.1094/mpmi.1998.11.1.71

Read article for free, from open access legal sources, via Unpaywall: https://apsjournals.apsnet.org/doi/pdf/10.1094/MPMI.1998.11.1.71

References

Articles referenced by this article (17)

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Use of green fluorescent protein to detect expression of nif genes of Azoarcus sp. BH72, a grass-associated diazotroph, on rice roots.

Affiliations

Authors

Abstract

Full text links

References

FACS-optimized mutants of the green fluorescent protein (GFP).

A transposon for green fluorescent protein transcriptional fusions: application for bacterial transport experiments.

Bipolar localization of the replication origin regions of chromosomes in vegetative and sporulating cells of B. subtilis.

Acetobacter diazotrophicus sp. nov., a Nitrogen-Fixing Acetic Acid Bacterium Associated with Sugarcane

Induction of complex intracytoplasmic membranes related to nitrogen fixation in Azoarcus sp. BH72.

Green fluorescent protein as a new expression marker in mycobacteria.

A taxonomic study of the Spirillum lipoferum group, with descriptions of a new genus, Azospirillum gen. nov. and two species, Azospirillum lipoferum (Beijerinck) comb. nov. and Azospirillum brasilense sp. nov.

A review of issues related to measuring colonization of plant roots by bacteria

Quantification of biological nitrogen fixation associated with sugar cane using a 15N aided nitrogen balance.

Agronomic applications of Azospirillum: an evaluation of 20 years worldwide field inoculation

Citations & impact

Impact metrics

Citations of article over time

Alternative metrics

Article citations

Improvement of morphophysiological and anatomical attributes of plants under abiotic stress conditions using plant growth-promoting bacteria and safety treatments.

Effects on gene expression during maize-Azospirillum interaction in the presence of a plant-specific inhibitor of indole-3-acetic acid production.

Gold-FISH enables targeted NanoSIMS analysis of plant-associated bacteria.

Elucidation of Essential Genes and Mutant Fitness during Adaptation toward Nitrogen Fixation Conditions in the Endophyte Azoarcus olearius BH72 Revealed by Tn-Seq.

RNA-Seq Provides New Insights into the Gene Expression Changes in Azoarcus olearius BH72 under Nitrogen-Deficient and Replete Conditions beyond the Nitrogen Fixation Process.

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Search life-sciences literature (45,104,206 articles, preprints and more)

Use of green fluorescent protein to detect expression of nif genes of Azoarcus sp. BH72, a grass-associated diazotroph, on rice roots.

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Affiliations

Authors

Abstract

Full text links

References

Acetobacter diazotrophicus sp. nov., a Nitrogen-Fixing Acetic Acid Bacterium Associated with Sugarcane

A review of issues related to measuring colonization of plant roots by bacteria

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