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Fig. 5

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SE-HPLC chromatogram (wavelength at 280 nm and 430 nm) of a Fe-DFO-N-suc-TFP ester chelator conjugated mAb before the subsequent Fe-removal and purification. The ratio between the Fe-DFO-N-suc-mAb and unbound Fe-DFO-N-suc TFP ester can be used to determine the chelator-to-mAb ratio

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