Fig. 7

DUXAP8 overexpression induces preeclampsia-like phenotypes in pregnant rats. A Schematic diagram of animal experimental design. B The expression of DUXAP8 in placenta was detected by RT-qPCR (n=6). C Systolic blood pressure (SBP) and Diastolic blood pressure (DBP) of pregnant rats were measured by tail-cuff system (GD9 and GD15) or carotid artery incubation (GD19) (n=6); 24-hour proteinuria levels were detected by a protein assay kit(n=6). D Placental weight of pregnant rats (n=6). E Placental morphological features were identified by Masson’s trichrome staining(n=6), and the histopathologic analysis of renal tissues was detected by periodic acid Schiff (n=6). F The expression of key signaling proteins (AKT, mTOR, p-AKT^Ser473, p-AKT^Thr308, p-mTOR^S2248), FAM134B and LC3 proteins were detected by Western blot (n=6). G The protein aggregation in placental trophoblast cells of pregnant rats was detected by Protein Aggresome detection kit (Scale bar =50 μm) (DAPI: nucleus; Aggresome: protein aggregates). Data are presented as mean±SD, statistical significance was evaluated using the t-test or Mann-Whitney for 2 comparisons and two-way ANOVA for multiple comparison