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New families in the classification of glycosyl hydrolases based on amino acid sequence similarities.

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  • 1. Centre de Recherches sur les Macromolécules Végétales, C.N.R.S., Grenoble, France.
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    • Henrissat B 1
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The Biochemical Journal, 01 Aug 1993, 293 ( Pt 3):781-788
https://doi.org/10.1042/bj2930781 PMID: 8352747 PMCID: PMC1134435

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Abstract 

301 glycosyl hydrolases and related enzymes corresponding to 39 EC entries of the I.U.B. classification system have been classified into 35 families on the basis of amino-acid-sequence similarities [Henrissat (1991) Biochem. J. 280, 309-316]. Approximately half of the families were found to be monospecific (containing only one EC number), whereas the other half were found to be polyspecific (containing at least two EC numbers). A > 60% increase in sequence data for glycosyl hydrolases (181 additional enzymes or enzyme domains sequences have since become available) allowed us to update the classification not only by the addition of more members to already identified families, but also by the finding of ten new families. On the basis of a comparison of 482 sequences corresponding to 52 EC entries, 45 families, out of which 22 are polyspecific, can now be defined. This classification has been implemented in the SWISS-PROT protein sequence data bank.

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Biochem J. 1993 Aug 1; 293(Pt 3): 781–788.
PMCID: PMC1134435
PMID: 8352747

New families in the classification of glycosyl hydrolases based on amino acid sequence similarities.

B Henrissat and A Bairoch
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Abstract

301 glycosyl hydrolases and related enzymes corresponding to 39 EC entries of the I.U.B. classification system have been classified into 35 families on the basis of amino-acid-sequence similarities [Henrissat (1991) Biochem. J. 280, 309-316]. Approximately half of the families were found to be monospecific (containing only one EC number), whereas the other half were found to be polyspecific (containing at least two EC numbers). A > 60% increase in sequence data for glycosyl hydrolases (181 additional enzymes or enzyme domains sequences have since become available) allowed us to update the classification not only by the addition of more members to already identified families, but also by the finding of ten new families. On the basis of a comparison of 482 sequences corresponding to 52 EC entries, 45 families, out of which 22 are polyspecific, can now be defined. This classification has been implemented in the SWISS-PROT protein sequence data bank.

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Selected References

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