Europe PMC

This website requires cookies, and the limited processing of your personal data in order to function. By using the site you are agreeing to this as outlined in our privacy notice and cookie policy.

Stimulation of prostaglandin production through purinoceptors on cultured porcine endothelial cells.

The Biochemical Journal, 01 Jul 1983, 214(1):273-276
https://doi.org/10.1042/bj2140273 PMID: 6311177 PMCID: PMC1152238

Free full text in Europe PMC

Abstract 

ATP (approx. 1-300 microM) induces dose-dependent prostacyclin secretion from perfused columns of microcarrier beads with cultured endothelial monolayers attached. The response is transient, shows little tachyphylaxis and can reach approx. 100 times control values. 2-Methylthio-ATP is more potent, ADP slightly less potent, AMP much less potent and adenosine is ineffective. These results are consistent with the presence of a purinoceptor on endothelium linked to the prostacyclin synthetic pathway.

Free full text 

Logo of biochemjLink to Publisher's site
Biochem J. 1983 Jul 15; 214(1): 273–276.
PMCID: PMC1152238
PMID: 6311177

Stimulation of prostaglandin production through purinoceptors on cultured porcine endothelial cells.

J D Pearson, L L Slakey, and J L Gordon
Copyright and License information Disclaimer

Abstract

ATP (approx. 1-300 microM) induces dose-dependent prostacyclin secretion from perfused columns of microcarrier beads with cultured endothelial monolayers attached. The response is transient, shows little tachyphylaxis and can reach approx. 100 times control values. 2-Methylthio-ATP is more potent, ADP slightly less potent, AMP much less potent and adenosine is ineffective. These results are consistent with the presence of a purinoceptor on endothelium linked to the prostacyclin synthetic pathway.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (489K), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.
 
icon of scanned page 273
273
icon of scanned page 274
274
icon of scanned page 275
275
icon of scanned page 276
276
 

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Ager A, Gordon JL, Moncada S, Pearson JD, Salmon JA, Trevethick MA. Effects of isolation and culture on prostaglandin synthesis by porcine aortic endothelial and smooth muscle cells. J Cell Physiol. 1982 Jan;110(1):9–16. [Abstract] [Google Scholar]
  • Blair IA, Barrow SE, Waddell KA, Lewis PJ, Dollery CT. Prostacyclin is not a circulating hormone in man. Prostaglandins. 1982 Apr;23(4):579–589. [Abstract] [Google Scholar]
  • Busch C, Owen WG. Identification in vitro of an endothelial cell surface cofactor for antithrombin III. Parallel studies with isolated perfused rat hearts and microcarrier cultures of bovine endothelium. J Clin Invest. 1982 Mar;69(3):726–729. [Europe PMC free article] [Abstract] [Google Scholar]
  • Davies PF. Microcarrier culture of vascular endothelial cells on solid plastic beads. Exp Cell Res. 1981 Aug;134(2):367–376. [Abstract] [Google Scholar]
  • De Mey JG, Vanhoutte PM. Role of the intima in cholinergic and purinergic relaxation of isolated canine femoral arteries. J Physiol. 1981 Jul;316:347–355. [Abstract] [Google Scholar]
  • Larsen M, Willett R, Yount RG. Imidodiphosphate and pyrophosphate: possible biological significance of similar structures. Science. 1969 Dec 19;166(3912):1510–1511. [Abstract] [Google Scholar]
  • Needleman P, Minkes MS, Douglas JR., Jr Stimulation of prostaglandin biosynthesis by adenine nucleotides. Profile of prostaglandin release by perfused organs. Circ Res. 1974 Apr;34(4):455–460. [Abstract] [Google Scholar]
  • Pearson JD, Gordon JL. Vascular endothelial and smooth muscle cells in culture selectively release adenine nucleotides. Nature. 1979 Oct 4;281(5730):384–386. [Abstract] [Google Scholar]
  • Pearson JD, Carleton JS, Hutchings A, Gordon JL. Uptake and metabolism of adenosine by pig aortic endothelial and smooth-muscle cells in culture. Biochem J. 1978 Feb 15;170(2):265–271. [Europe PMC free article] [Abstract] [Google Scholar]
  • Pearson JD, Carleton JS, Gordon JL. Metabolism of adenine nucleotides by ectoenzymes of vascular endothelial and smooth-muscle cells in culture. Biochem J. 1980 Aug 15;190(2):421–429. [Europe PMC free article] [Abstract] [Google Scholar]
  • Pearson JD, Carleton JS, Hutchings A. Prostacyclin release stimulated by thrombin or bradykinin in porcine endothelial cells cultured from aorta and umbilical vein. Thromb Res. 1983 Jan 15;29(2):115–124. [Abstract] [Google Scholar]
  • Schwartzman M, Raz A. Purinergic vs peptidergic stimulation of lipolysis and prostaglandin generation in the perfused rabbit kidney. Biochem Pharmacol. 1982 Aug 1;31(15):2453–2458. [Abstract] [Google Scholar]
  • Weksler BB, Ley CW, Jaffe EA. Stimulation of endothelial cell prostacyclin production by thrombin, trypsin, and the ionophore A 23187. J Clin Invest. 1978 Nov;62(5):923–930. [Europe PMC free article] [Abstract] [Google Scholar]
Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

Full text links 

Read article at publisher's site: https://doi.org/10.1042/bj2140273

Read article for free, from open access legal sources, via Unpaywall: https://europepmc.org/articles/pmc1152238?pdf=renderUnpaywall

Citations & impact 

Impact metrics

118
Citations
Jump to Citations

Citations of article over time

Article citations

Go to all (118) article citations

Data 

Data behind the article

This data has been text mined from the article, or deposited into data resources.

BioStudies: supplemental material and supporting data

Similar Articles 

To arrive at the top five similar articles we use a word-weighted algorithm to compare words from the Title and Abstract of each citation.