Europe PMC

This website requires cookies, and the limited processing of your personal data in order to function. By using the site you are agreeing to this as outlined in our privacy notice and cookie policy.

SGS1, a homologue of the Bloom's and Werner's syndrome genes, is required for maintenance of genome stability in Saccharomyces cerevisiae.

Author information

Affiliations

  • 1. Imperial Cancer Research Fund Laboratories, University of Oxford, John Radcliffe Hospital, United Kingdom.
      Authors
    • Watt PM 1
    (1 author)

Genetics, 01 Nov 1996, 144(3):935-945
https://doi.org/10.1093/genetics/144.3.935 PMID: 8913739 PMCID: PMC1207633

Free full text in Europe PMC

Abstract 

The Saccharomyces cerevisiae SGS1 gene is homologous to Escherichia coli RecQ and the human BLM and WRN proteins that are defective in the cancer-prone disorder Bloom's syndrome and the premature aging disorder Werner's syndrome, respectively. While recQ mutants are deficient in conjugational recombination and DNA repair, Bloom's syndrome cell lines show hyperrecombination. Bloom's and Werner's syndrome cell lines both exhibit chromosomal instability, sgs1 delta strains show mitotic hyperrecombination, as do Bloom's cells. This was manifested as an increase in the frequency of interchromosomal homologous recombination, intrachromosomal excision recombination, and ectopic recombination. Hyperrecombination was partially independent of both RAD52 and RAD1. Meiotic recombination was not increased in sgs1 delta mutants, although meiosis I chromosome missegregation has been shown to be elevated sgs1 delta suppresses the slow growth of a top3 delta strain lacking topoisomerase III. Although there was an increase in subtelomeric Y' instability in sgs1 delta strains due to hyperrecombination, no evidence was found for an increase in the instability of terminal telomeric sequences in a top3 delta or a sgs1 delta strain. This contrasts with the telomere maintenance defects of Werner's patients. We conclude that the SGS1 gene product is involved in the maintenance of genome stability in S. cerevisiae.

Free full text 

Logo of geneticsLink to Publisher's site
Genetics. 1996 Nov; 144(3): 935–945.
PMCID: PMC1207633
PMID: 8913739

Sgs1, a Homologue of the Bloom's and Werner's Syndrome Genes, Is Required for Maintenance of Genome Stability in Saccharomyces Cerevisiae

P. M. Watt, I. D. Hickson, R. H. Borts, and E. J. Louis
Author information Copyright and License information Disclaimer

Abstract

The Saccharomyces cerevisiae SGS1 gene is homologous to Escherichia coli RecQ and the human BLM and WRN proteins that are defective in the cancer-prone disorder Bloom's syndrome and the premature aging disorder Werner's syndrome, respectively. While recQ mutants are deficient in conjugational recombination and DNA repair, Bloom's syndrome cell lines show hyperrecombination. Bloom's and Werner's syndrome cell lines both exhibit chromosomal instability. sgs1Δ strains show mitotic hyperrecombination, as do Bloom's cells. This was manifested as an increase in the frequency of interchromosomal homologous recombination, intrachromosomal excision recombination, and ectopic recombination. Hyperrecombination was partially independent of both RAD52 and RAD1. Meiotic recombination was not increased in sgs1Δ mutants, although meiosis I chromosome missegregation has been shown to be elevated. sgs1Δ suppresses the slow growth of a top3Δ strain lacking topoisomerase III. Although there was an increase in subtelomeric Y' instability in sgs1Δ strains due to hyperrecombination, no evidence was found for an increase in the instability of terminal telomeric sequences in a top3Δ or a sgs1Δ strain. This contrasts with the telomere maintenance defects of Werner's patients. We conclude that the SGS1 gene product is involved in the maintenance of genome stability in S. cerevisiae.

Full Text

The Full Text of this article is available as a PDF (3.7M).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Adams DE, Bliska JB, Cozzarelli NR. Cre-lox recombination in Escherichia coli cells. Mechanistic differences from the in vitro reaction. J Mol Biol. 1992 Aug 5;226(3):661–673. [Abstract] [Google Scholar]
  • Bailis AM, Arthur L, Rothstein R. Genome rearrangement in top3 mutants of Saccharomyces cerevisiae requires a functional RAD1 excision repair gene. Mol Cell Biol. 1992 Nov;12(11):4988–4993. [Europe PMC free article] [Abstract] [Google Scholar]
  • Bierne H, Michel B. When replication forks stop. Mol Microbiol. 1994 Jul;13(1):17–23. [Abstract] [Google Scholar]
  • Bishop DK, Park D, Xu L, Kleckner N. DMC1: a meiosis-specific yeast homolog of E. coli recA required for recombination, synaptonemal complex formation, and cell cycle progression. Cell. 1992 May 1;69(3):439–456. [Abstract] [Google Scholar]
  • Borts RH, Haber JE. Meiotic recombination in yeast: alteration by multiple heterozygosities. Science. 1987 Sep 18;237(4821):1459–1465. [Abstract] [Google Scholar]
  • Borts RH, Lichten M, Haber JE. Analysis of meiosis-defective mutations in yeast by physical monitoring of recombination. Genetics. 1986 Jul;113(3):551–567. [Europe PMC free article] [Abstract] [Google Scholar]
  • Christman MF, Dietrich FS, Fink GR. Mitotic recombination in the rDNA of S. cerevisiae is suppressed by the combined action of DNA topoisomerases I and II. Cell. 1988 Nov 4;55(3):413–425. [Abstract] [Google Scholar]
  • Ellis NA, Groden J, Ye TZ, Straughen J, Lennon DJ, Ciocci S, Proytcheva M, German J. The Bloom's syndrome gene product is homologous to RecQ helicases. Cell. 1995 Nov 17;83(4):655–666. [Abstract] [Google Scholar]
  • Game JC, Zamb TJ, Braun RJ, Resnick M, Roth RM. The Role of Radiation (rad) Genes in Meiotic Recombination in Yeast. Genetics. 1980 Jan;94(1):51–68. [Europe PMC free article] [Abstract] [Google Scholar]
  • Gangloff S, McDonald JP, Bendixen C, Arthur L, Rothstein R. The yeast type I topoisomerase Top3 interacts with Sgs1, a DNA helicase homolog: a potential eukaryotic reverse gyrase. Mol Cell Biol. 1994 Dec;14(12):8391–8398. [Europe PMC free article] [Abstract] [Google Scholar]
  • Gietz D, St Jean A, Woods RA, Schiestl RH. Improved method for high efficiency transformation of intact yeast cells. Nucleic Acids Res. 1992 Mar 25;20(6):1425–1425. [Europe PMC free article] [Abstract] [Google Scholar]
  • Gorbalenya AE, Koonin EV, Donchenko AP, Blinov VM. Two related superfamilies of putative helicases involved in replication, recombination, repair and expression of DNA and RNA genomes. Nucleic Acids Res. 1989 Jun 26;17(12):4713–4730. [Europe PMC free article] [Abstract] [Google Scholar]
  • Keil RL, McWilliams AD. A gene with specific and global effects on recombination of sequences from tandemly repeated genes in Saccharomyces cerevisiae. Genetics. 1993 Nov;135(3):711–718. [Europe PMC free article] [Abstract] [Google Scholar]
  • Kim RA, Wang JC. A subthreshold level of DNA topoisomerases leads to the excision of yeast rDNA as extrachromosomal rings. Cell. 1989 Jun 16;57(6):975–985. [Abstract] [Google Scholar]
  • Koonin EV. Similarities in RNA helicases. Nature. 1991 Jul 25;352(6333):290–290. [Abstract] [Google Scholar]
  • Liefshitz B, Parket A, Maya R, Kupiec M. The role of DNA repair genes in recombination between repeated sequences in yeast. Genetics. 1995 Aug;140(4):1199–1211. [Europe PMC free article] [Abstract] [Google Scholar]
  • Louis EJ, Haber JE. Nonrecombinant meiosis I nondisjunction in Saccharomyces cerevisiae induced by tRNA ochre suppressors. Genetics. 1989 Sep;123(1):81–95. [Europe PMC free article] [Abstract] [Google Scholar]
  • Louis EJ, Haber JE. Mitotic recombination among subtelomeric Y' repeats in Saccharomyces cerevisiae. Genetics. 1990 Mar;124(3):547–559. [Europe PMC free article] [Abstract] [Google Scholar]
  • Louis EJ, Naumova ES, Lee A, Naumov G, Haber JE. The chromosome end in yeast: its mosaic nature and influence on recombinational dynamics. Genetics. 1994 Mar;136(3):789–802. [Europe PMC free article] [Abstract] [Google Scholar]
  • Luisi-DeLuca C, Lovett ST, Kolodner RD. Genetic and physical analysis of plasmid recombination in recB recC sbcB and recB recC sbcA Escherichia coli K-12 mutants. Genetics. 1989 Jun;122(2):269–278. [Europe PMC free article] [Abstract] [Google Scholar]
  • Lundblad V, Szostak JW. A mutant with a defect in telomere elongation leads to senescence in yeast. Cell. 1989 May 19;57(4):633–643. [Abstract] [Google Scholar]
  • Nakayama H, Nakayama K, Nakayama R, Irino N, Nakayama Y, Hanawalt PC. Isolation and genetic characterization of a thymineless death-resistant mutant of Escherichia coli K12: identification of a new mutation (recQ1) that blocks the RecF recombination pathway. Mol Gen Genet. 1984;195(3):474–480. [Abstract] [Google Scholar]
  • Nakayama K, Irino N, Nakayama H. The recQ gene of Escherichia coli K12: molecular cloning and isolation of insertion mutants. Mol Gen Genet. 1985;200(2):266–271. [Abstract] [Google Scholar]
  • Puranam KL, Blackshear PJ. Cloning and characterization of RECQL, a potential human homologue of the Escherichia coli DNA helicase RecQ. J Biol Chem. 1994 Nov 25;269(47):29838–29845. [Abstract] [Google Scholar]
  • Rothstein RJ. One-step gene disruption in yeast. Methods Enzymol. 1983;101:202–211. [Abstract] [Google Scholar]
  • Seki M, Miyazawa H, Tada S, Yanagisawa J, Yamaoka T, Hoshino S, Ozawa K, Eki T, Nogami M, Okumura K, et al. Molecular cloning of cDNA encoding human DNA helicase Q1 which has homology to Escherichia coli Rec Q helicase and localization of the gene at chromosome 12p12. Nucleic Acids Res. 1994 Nov 11;22(22):4566–4573. [Europe PMC free article] [Abstract] [Google Scholar]
  • Shinohara A, Ogawa H, Ogawa T. Rad51 protein involved in repair and recombination in S. cerevisiae is a RecA-like protein. Cell. 1992 May 1;69(3):457–470. [Abstract] [Google Scholar]
  • Thomas BJ, Rothstein R. The genetic control of direct-repeat recombination in Saccharomyces: the effect of rad52 and rad1 on mitotic recombination at GAL10, a transcriptionally regulated gene. Genetics. 1989 Dec;123(4):725–738. [Europe PMC free article] [Abstract] [Google Scholar]
  • Wallis JW, Chrebet G, Brodsky G, Rolfe M, Rothstein R. A hyper-recombination mutation in S. cerevisiae identifies a novel eukaryotic topoisomerase. Cell. 1989 Jul 28;58(2):409–419. [Abstract] [Google Scholar]
  • Wang JC, Caron PR, Kim RA. The role of DNA topoisomerases in recombination and genome stability: a double-edged sword? Cell. 1990 Aug 10;62(3):403–406. [Abstract] [Google Scholar]
  • Watt PM, Hickson ID. Failure to unwind causes cancer. Genome stability. Curr Biol. 1996 Mar 1;6(3):265–267. [Abstract] [Google Scholar]
  • Watt PM, Louis EJ, Borts RH, Hickson ID. Sgs1: a eukaryotic homolog of E. coli RecQ that interacts with topoisomerase II in vivo and is required for faithful chromosome segregation. Cell. 1995 Apr 21;81(2):253–260. [Abstract] [Google Scholar]
  • Wierdl M, Greene CN, Datta A, Jinks-Robertson S, Petes TD. Destabilization of simple repetitive DNA sequences by transcription in yeast. Genetics. 1996 Jun;143(2):713–721. [Europe PMC free article] [Abstract] [Google Scholar]
  • West SC. Enzymes and molecular mechanisms of genetic recombination. Annu Rev Biochem. 1992;61:603–640. [Abstract] [Google Scholar]
  • Yu CE, Oshima J, Fu YH, Wijsman EM, Hisama F, Alisch R, Matthews S, Nakura J, Miki T, Ouais S, et al. Positional cloning of the Werner's syndrome gene. Science. 1996 Apr 12;272(5259):258–262. [Abstract] [Google Scholar]
Articles from Genetics are provided here courtesy of Oxford University Press

Full text links 

Read article at publisher's site: https://doi.org/10.1093/genetics/144.3.935

Read article for free, from open access legal sources, via Unpaywall: https://academic.oup.com/genetics/article-pdf/144/3/935/34667912/genetics0935.pdfUnpaywall

Subscription required at intl.genetics.org
http://intl.genetics.org/cgi/reprint/144/3/935.pdf

Free to read at intl.genetics.org
http://intl.genetics.org/cgi/content/abstract/144/3/935

Citations & impact 

Impact metrics

253
Citations
Jump to Citations
1
Data citation
Jump to Data

Citations of article over time

Alternative metrics

Altmetric item for https://www.altmetric.com/details/23961989
Altmetric
Discover the attention surrounding your research
https://www.altmetric.com/details/23961989

Smart citations by scite.ai
Smart citations by scite.ai include citation statements extracted from the full text of the citing article. The number of the statements may be higher than the number of citations provided by EuropePMC if one paper cites another multiple times or lower if scite has not yet processed some of the citing articles.
Explore citation contexts and check if this article has been supported or disputed.
https://scite.ai/reports/10.1093/genetics/144.3.935

Supporting
Mentioning
Contrasting
2
48
0

Article citations

Go to all (253) article citations

Data 

Similar Articles 

To arrive at the top five similar articles we use a word-weighted algorithm to compare words from the Title and Abstract of each citation.

Funding 

Funders who supported this work.

Wellcome Trust