Figure 4

In situ hybridization for VEGF₁₈₉ mRNA in human endometrium. Sections were hybridized with a radiolabeled antisense V₁₈₉ probe (A–D) or a probe for all VEGF transcripts (E and F). (A and B) Late secretory phase (day 28), strong V₁₈₉ expression in decidual cells. (C and D) Early pregnancy, stromal cells near a blood vessel (v); dark-field in A and C and bright-field in B and D. (Inset) No staining is detected with the corresponding sense probe on sections adjacent to those shown in panels C and D. (E and F) Proliferative (day 11) and secretory (day 25) phase, expression of all VEGF mRNA by using exon 4 antisense probe is moderate in stromal cells. Hematoxylin/eosin counterstaining. (Magnification, ×400.)