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Figure 6

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MUC1 internalization is inhibited by hypertonic media. (A) CHO and ldlD cells expressing MUC1 were pulse labeled for 30 min and chased for 90 min. GalNAc (1000 μM) was included in the starvation, pulse, and chase media for the ldlD cells. After biotinylation on ice, cells were rapidly warmed to 37°C in the presence of normal or hypertonic media (supplemented with 0.45 M sucrose) for the indicated times. The level of internalized MUC1 was determined as described in MATERIALS AND METHODS and the legend to Figure ​Figure4.4. (B) ldlD cells expressing MUC1 were preincubated for 2 h in media with 1000 μM GalNAc, with or without 100 μM Gal. Endocytosis of the fluid phase marker HRP, carried out in the presence (+ sucrose) or absence of hypertonic media, was determined as described in MATERIALS AND METHODS.

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