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Table 1
Characterization of the Rac second effector mutants
| Assay | Mutants
| ||||
|---|---|---|---|---|---|
| L61Rac | A147 A148 | A162 A163 | A166 A167 | A170 A171 | |
| JNK activation | + | + | + | + | − |
| Target binding | |||||
 RhoGAP | + | + | + | + | + |
| PAK | + | + | + | + | + |
| ROK-α | + | + | + | + | + |
| MLK2 | + | + | + | − | − |
| MLK3 | + | +/− | +/− | − | − |
| IQGAP2 | + | + | + | + | + |
| Empty vector | − | − | − | − | − |
L61Rac and the double alanine Rac mutants were evaluated for activation of the JNK pathway and their binding with distinct targets in the yeast two-hybrid assay. The Rac targets tested are RhoGAP, PAK, ROK-α, MLK2, MLK3 and IQGAP2: (−), negative; (+), positive; (+/−), weakly positive as revealed by growth in 3AT plates (see MATERIALS AND METHODS for details). Empty vector was used as a negative control.