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Fine regulation of cI857-controlled gene expression in continuous culture of recombinant Escherichia coli by temperature.

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  • 1. Institut de Biologia Fonamental, Universitat Autònoma de Barcelona, Bellaterra, Spain.
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    • Villaverde A 1
    (1 author)

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Applied and Environmental Microbiology, 01 Oct 1993, 59(10):3485-3487
https://doi.org/10.1128/aem.59.10.3485-3487.1993 PMID: 8250569 PMCID: PMC182479

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Abstract 

The expression at different temperatures of the lacZ gene, which is controlled by the lambda pL and pR tandem promoters and the cI857 temperature-sensitive repressor, was studied in Escherichia coli continuous cultures. At temperatures between 30 and 42 degrees C, beta-galactosidase activity behaved according to an exponential equation. By inducing a culture at a temperature within this range, predefined, nearly constant submaximal levels of gene expression and recombinant product yield can be obtained.

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Appl Environ Microbiol. 1993 Oct; 59(10): 3485–3487.
PMCID: PMC182479
PMID: 8250569

Fine regulation of cI857-controlled gene expression in continuous culture of recombinant Escherichia coli by temperature.

A Villaverde, A Benito, E Viaplana, and R Cubarsi
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Abstract

The expression at different temperatures of the lacZ gene, which is controlled by the lambda pL and pR tandem promoters and the cI857 temperature-sensitive repressor, was studied in Escherichia coli continuous cultures. At temperatures between 30 and 42 degrees C, beta-galactosidase activity behaved according to an exponential equation. By inducing a culture at a temperature within this range, predefined, nearly constant submaximal levels of gene expression and recombinant product yield can be obtained.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Benito A, Valero F, Lafuente J, Vidal M, Cairo J, Solà C, Villaverde A. Uses of beta-galactosidase tag in on-line monitoring production of fusion proteins and gene expression in Escherichia coli. Enzyme Microb Technol. 1993 Jan;15(1):66–71. [Abstract] [Google Scholar]
  • Benito A, Vidal M, Villaverde A. Enhanced production of pL-controlled recombinant proteins and plasmid stability in Escherichia coli RecA+ strains. J Biotechnol. 1993 Jun;29(3):299–306. [Abstract] [Google Scholar]
  • Casadaban MJ, Chou J, Cohen SN. In vitro gene fusions that join an enzymatically active beta-galactosidase segment to amino-terminal fragments of exogenous proteins: Escherichia coli plasmid vectors for the detection and cloning of translational initiation signals. J Bacteriol. 1980 Aug;143(2):971–980. [Europe PMC free article] [Abstract] [Google Scholar]
  • Gaitanaris GA, Papavassiliou AG, Rubock P, Silverstein SJ, Gottesman ME. Renaturation of denatured lambda repressor requires heat shock proteins. Cell. 1990 Jun 15;61(6):1013–1020. [Abstract] [Google Scholar]
  • Hortacsu A, Ryu DD. Optimal temperature control policy for a two-stage recombinant fermentation process. Biotechnol Prog. 1990 Nov-Dec;6(6):403–407. [Abstract] [Google Scholar]
  • Kucharczyk K, Laskowska E, Taylor A. Response of Escherichia coli cell membranes to induction of lambda cl857 prophage by heat shock. Mol Microbiol. 1991 Dec;5(12):2935–2945. [Abstract] [Google Scholar]
  • Lieb M. Studies of heat-inducible lambda bacteriophage. I. Order of genetic sites and properties of mutant prophages. J Mol Biol. 1966 Mar;16(1):149–163. [Abstract] [Google Scholar]
  • Lowman HB, Bina M. Temperature-mediated regulation and downstream inducible selection for controlling gene expression from the bacteriophage lambda pL promoter. Gene. 1990 Nov 30;96(1):133–136. [Abstract] [Google Scholar]
  • Mott JE, Grant RA, Ho YS, Platt T. Maximizing gene expression from plasmid vectors containing the lambda PL promoter: strategies for overproducing transcription termination factor rho. Proc Natl Acad Sci U S A. 1985 Jan;82(1):88–92. [Europe PMC free article] [Abstract] [Google Scholar]
  • Poindexter K, Gayle RB., 3rd Induction of recombinant gene expression in Escherichia coli using an alkaline pH shift. Gene. 1991 Jan 2;97(1):125–130. [Abstract] [Google Scholar]
  • Schein CH. Optimizing protein folding to the native state in bacteria. Curr Opin Biotechnol. 1991 Oct;2(5):746–750. [Abstract] [Google Scholar]
  • Schauder B, Blöcker H, Frank R, McCarthy JE. Inducible expression vectors incorporating the Escherichia coli atpE translational initiation region. Gene. 1987;52(2-3):279–283. [Abstract] [Google Scholar]
  • Strandberg L, Enfors SO. Factors influencing inclusion body formation in the production of a fused protein in Escherichia coli. Appl Environ Microbiol. 1991 Jun;57(6):1669–1674. [Europe PMC free article] [Abstract] [Google Scholar]
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