Europe PMC

This website requires cookies, and the limited processing of your personal data in order to function. By using the site you are agreeing to this as outlined in our privacy notice and cookie policy.

Tangential flow filtration and preliminary phylogenetic analysis of marine picoplankton.

Author information

Affiliations

  • 1. Department of Biology, Indiana University, Bloomington 47405.
      Authors
    • Giovannoni SJ 1
    (1 author)

ORCIDs linked to this article

Applied and Environmental Microbiology, 01 Aug 1990, 56(8):2572-2575
https://doi.org/10.1128/aem.56.8.2572-2575.1990 PMID: 2403262 PMCID: PMC184769

Free full text in Europe PMC

Abstract 

A procedure was developed for harvesting gram quantities of microbial biomass from oligotrophic waters, when mixed populations are present in low abundance. Picoplankton from Atlantic Ocean (Hydrostation S, Sargasso Sea) and Pacific Ocean (Aloha Station) sites were collected in a three-stage process: (i) collection of seawater through an intake covered with 10-microns-pore Nytex; (ii) concentration by a tangential flow filtration device equipped with 10 ft2 (0.929 m2) of 0.1-micron-pore fluorocarbon membrane; (iii) collection of cells from concentrate by centrifugation. The overall efficiency of picoplankton recovery was at least 37%. The cellular morphotypes recovered matched those of the original population. DNA was prepared from frozen cell pellets by enzymatic digestion, solvent extraction, and isopycnic centrifugation. As indicated by the binding of kingdom-specific hybridization probes to the purified DNA, the Sargasso Sea picoplankton in this collection were largely eubacteria.

Free full text 

Logo of aemLink to Publisher's site
Appl Environ Microbiol. 1990 Aug; 56(8): 2572–2575.
PMCID: PMC184769
PMID: 2403262

Tangential flow filtration and preliminary phylogenetic analysis of marine picoplankton.

S J Giovannoni, E F DeLong, T M Schmidt, and N R Pace
Author information Copyright and License information Disclaimer

Abstract

A procedure was developed for harvesting gram quantities of microbial biomass from oligotrophic waters, when mixed populations are present in low abundance. Picoplankton from Atlantic Ocean (Hydrostation S, Sargasso Sea) and Pacific Ocean (Aloha Station) sites were collected in a three-stage process: (i) collection of seawater through an intake covered with 10-microns-pore Nytex; (ii) concentration by a tangential flow filtration device equipped with 10 ft2 (0.929 m2) of 0.1-micron-pore fluorocarbon membrane; (iii) collection of cells from concentrate by centrifugation. The overall efficiency of picoplankton recovery was at least 37%. The cellular morphotypes recovered matched those of the original population. DNA was prepared from frozen cell pellets by enzymatic digestion, solvent extraction, and isopycnic centrifugation. As indicated by the binding of kingdom-specific hybridization probes to the purified DNA, the Sargasso Sea picoplankton in this collection were largely eubacteria.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (1.2M), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.
 
icon of scanned page 2572
2572
icon of scanned page 2573
2573
icon of scanned page 2574
2574
icon of scanned page 2575
2575
 

Images in this article

Image
Image
on p.2573
Image
Image
on p.2574
Click on the image to see a larger version.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Ferguson RL, Buckley EN, Palumbo AV. Response of marine bacterioplankton to differential filtration and confinement. Appl Environ Microbiol. 1984 Jan;47(1):49–55. [Europe PMC free article] [Abstract] [Google Scholar]
  • Fuhrman JA, Comeau DE, Hagström A, Chan AM. Extraction from natural planktonic microorganisms of DNA suitable for molecular biological studies. Appl Environ Microbiol. 1988 Jun;54(6):1426–1429. [Europe PMC free article] [Abstract] [Google Scholar]
  • Giovannoni SJ, DeLong EF, Olsen GJ, Pace NR. Phylogenetic group-specific oligodeoxynucleotide probes for identification of single microbial cells. J Bacteriol. 1988 Feb;170(2):720–726. [Europe PMC free article] [Abstract] [Google Scholar]
  • Hobbie JE, Daley RJ, Jasper S. Use of nuclepore filters for counting bacteria by fluorescence microscopy. Appl Environ Microbiol. 1977 May;33(5):1225–1228. [Europe PMC free article] [Abstract] [Google Scholar]
  • Kogure K, Simidu U, Taga N. A tentative direct microscopic method for counting living marine bacteria. Can J Microbiol. 1979 Mar;25(3):415–420. [Abstract] [Google Scholar]
  • Lee S, Fuhrman JA. Relationships between Biovolume and Biomass of Naturally Derived Marine Bacterioplankton. Appl Environ Microbiol. 1987 Jun;53(6):1298–1303. [Europe PMC free article] [Abstract] [Google Scholar]
  • Olsen GJ, Lane DJ, Giovannoni SJ, Pace NR, Stahl DA. Microbial ecology and evolution: a ribosomal RNA approach. Annu Rev Microbiol. 1986;40:337–365. [Abstract] [Google Scholar]
  • Sgaramella V, Khorana HG. CXII. Total synthesis of the structural gene for an alanine transfer RNA from yeast. Enzymic joining of the chemically synthesized polydeoxynucleotides to form the DNA duplex representing nucleotide sequence 1 to 20. J Mol Biol. 1972 Dec 28;72(2):427–444. [Abstract] [Google Scholar]
  • Somerville CC, Knight IT, Straube WL, Colwell RR. Simple, rapid method for direct isolation of nucleic acids from aquatic environments. Appl Environ Microbiol. 1989 Mar;55(3):548–554. [Europe PMC free article] [Abstract] [Google Scholar]
  • Watson SW, Novitsky TJ, Quinby HL, Valois FW. Determination of bacterial number and biomass in the marine environment. Appl Environ Microbiol. 1977 Apr;33(4):940–946. [Europe PMC free article] [Abstract] [Google Scholar]
  • Woese CR. Bacterial evolution. Microbiol Rev. 1987 Jun;51(2):221–271. [Europe PMC free article] [Abstract] [Google Scholar]
Articles from Applied and Environmental Microbiology are provided here courtesy of American Society for Microbiology (ASM)

Full text links 

Read article at publisher's site: https://doi.org/10.1128/aem.56.8.2572-2575.1990

Read article for free, from open access legal sources, via Unpaywall: https://aem.asm.org/content/aem/56/8/2572.full.pdfUnpaywall

Free to read at aem.asm.org
http://aem.asm.org/cgi/content/abstract/56/8/2572

Free after 4 months at aem.asm.org
http://aem.asm.org/cgi/reprint/56/8/2572

Citations & impact 

Impact metrics

97
Citations
Jump to Citations

Citations of article over time

Article citations

Go to all (97) article citations

Similar Articles 

To arrive at the top five similar articles we use a word-weighted algorithm to compare words from the Title and Abstract of each citation.

Funding 

Funders who supported this work.

NIGMS NIH HHS (1)