Cell death was assessed by trypan blue staining and expressed as a percentage of the total. (A) Cells transfected with CD8 alone or cotransfected with CD8 and LARD. (B) effects of crosslinking with an anti-Fas mAb. F, Fas; L, LARD; F/L, Fas/LARD chimera. (C) Expression of Fas on transfected cells analyzed by a fluorescence-activated cell sorter. These data are representative of three separate experiments.