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Identification and initial characterization of the eutF locus of Salmonella typhimurium.

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  • 1. Department of Bacteriology, University of Wisconsin, Madison 53706.
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    • O'Toole GA 1
    (1 author)

Journal of Bacteriology, 01 Aug 1991, 173(16):5168-5172
https://doi.org/10.1128/jb.173.16.5168-5172.1991 PMID: 1860825 PMCID: PMC208209

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Abstract 

We report the isolation and initial characterization of mutations in the newly described eutF locus of Salmonella typhimurium LT2. Mutations in eutF render a strain unable to utilize ethanolamine as a source of carbon and/or energy and impair growth on ethanolamine as a sole nitrogen source. Strains carrying eutF mutations exhibit a 2-order-of-magnitude decrease in transcription of the unlinked eutDEABCR operon (50 min), which codes for the enzymes needed to catabolize ethanolamine; have only 10% of the ethanolamine ammonia-lyase activity found in the wild type; and show a marked reduction in the rate of ethanolamine uptake. Deletion mapping and three-factor cross analysis results are consistent with the gene order cobA trp eutF tonB at 34 min on the linkage map. We discuss two possible roles for the EutF protein: (i) as an ethanolamine permease or (ii) as a transcription factor required for the expression of the eutDEABCR operon.

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J Bacteriol. 1991 Aug; 173(16): 5168–5172.
PMCID: PMC208209
PMID: 1860825

Identification and initial characterization of the eutF locus of Salmonella typhimurium.

G A O'Toole and J C Escalante-Semerena
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Abstract

We report the isolation and initial characterization of mutations in the newly described eutF locus of Salmonella typhimurium LT2. Mutations in eutF render a strain unable to utilize ethanolamine as a source of carbon and/or energy and impair growth on ethanolamine as a sole nitrogen source. Strains carrying eutF mutations exhibit a 2-order-of-magnitude decrease in transcription of the unlinked eutDEABCR operon (50 min), which codes for the enzymes needed to catabolize ethanolamine; have only 10% of the ethanolamine ammonia-lyase activity found in the wild type; and show a marked reduction in the rate of ethanolamine uptake. Deletion mapping and three-factor cross analysis results are consistent with the gene order cobA trp eutF tonB at 34 min on the linkage map. We discuss two possible roles for the EutF protein: (i) as an ethanolamine permease or (ii) as a transcription factor required for the expression of the eutDEABCR operon.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.
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