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Table 2.

Direct quantification of specific miRNAs per ES cell

miRNAJ1 (quant)J1 (reads)Dicer+/+ (quant)Dicer+/+ (reads)
miR15a290 ± 50175280 ± 20293
miR 15b950 ± 202,301970 ± 401,621
miR 161,130 ± 1402,0371090 ± 1201,199
miR 17–5p1,510 ± 1107951440 ± 1701,509
miR 19b2,140 ± 49014,7772340 ± 5503,918
miR 212,750 ± 4106,1721340 ± 4502,272
miR 30c250 ± 202,946220 ± 40379

The measured miRNA copy number is compared with the sequencing frequency per 130,000 reads in the J1 and Dicer+/+ libraries. Error is the SEM from 2 to 21 triplicate measurements. quant, quantified.