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Site-directed insertion and deletion mutagenesis with cloned fragments in Escherichia coli.

Journal of Bacteriology, 01 Mar 1985, 161(3):1219-1221
https://doi.org/10.1128/jb.161.3.1219-1221.1985 PMID: 2982787 PMCID: PMC215030

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Abstract 

A mutation of a cloned gene that has been made by introducing a transposon or some other selectable genetic determinant can be crossed into the gene's original replicon by linearizing the cloned DNA and transforming a recB recC sbcB mutant. A number of applications of this method are described with genes of either chromosomal or plasmid origin.

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J Bacteriol. 1985 Mar; 161(3): 1219–1221.
PMCID: PMC215030
PMID: 2982787

Site-directed insertion and deletion mutagenesis with cloned fragments in Escherichia coli.

S C Winans, S J Elledge, J H Krueger, and G C Walker
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Abstract

A mutation of a cloned gene that has been made by introducing a transposon or some other selectable genetic determinant can be crossed into the gene's original replicon by linearizing the cloned DNA and transforming a recB recC sbcB mutant. A number of applications of this method are described with genes of either chromosomal or plasmid origin.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.
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