Endothelial barrier disruption by VEGF-mediated Src activity potentiates tumor cell extravasation and metastasis

doi:10.1083/jcb.200408130

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J Cell Biol. 2004 Oct 25; 167(2): 223–229.

doi: 10.1083/jcb.200408130

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Disruption of cadherin-mediated adhesion enhances tumor cell extravasation. (A) Direct i.v. injection of VEGF induces rapid and transient dissociation of β-catenin from VE-cadherin in lung from wild-type, but not Yes-deficient mice, determined by immunoprecipitation and immunoblotting of mouse lung homogenates. Representative data from three experiments are shown. (B) Treating mice before tumor cell inoculation with VE-cadherin–disrupting antibody BV13 induces vascular permeability, facilitates CT26 tumor cell extravasation, and increases metastases. VE-cadherin antibody E4G10 which does not produce permeability has no impact on metastasis. Tumor burden represents increase in lung/heart weight ratio over control. * indicates P < 0.05; n = 8 each bar (A) and n = 4 each bar (B).

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