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Migration of monocytes across endothelium and passage through extracellular matrix involve separate molecular domains of PECAM-1.

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Affiliations

  • 1. Laboratory of Cellular Physiology and Immunology, Rockefeller University, New York 10021-6399, USA.
      Authors
    • Liao F 1
    (1 author)

The Journal of Experimental Medicine, 01 Nov 1995, 182(5):1337-1343
https://doi.org/10.1084/jem.182.5.1337 PMID: 7595204 PMCID: PMC2192210

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Abstract 

During the inflammatory response, the adhesion molecule PECAM plays a crucial role in transendothelial migration, the passage of leukocytes across endothelium. We report here an additional role for PECAM in the subsequent migration of monocytes through the subendothelial extracellular matrix. PECAM has six immunoglobulin (Ig) superfamily domains. Monoclonal antibodies whose epitopes map to domains 1 and/or 2 selectively block monocyte migration through the endothelial junction, whereas those that map to domain 6 block only the migration through the extracellular matrix, trapping the monocyte between the endothelium and its basal lamina. Therefore, transendothelial migration (diapedesis) and passage through extracellular matrix (interstitial migration) are distinct and separable phases of monocyte emigration. Furthermore, distinct and separate Ig domains of PECAM are involved in mediating these two steps.

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J Exp Med. 1995 Nov 1; 182(5): 1337–1343.
PMCID: PMC2192210
PMID: 7595204

Migration of monocytes across endothelium and passage through extracellular matrix involve separate molecular domains of PECAM-1

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Abstract

During the inflammatory response, the adhesion molecule PECAM plays a crucial role in transendothelial migration, the passage of leukocytes across endothelium. We report here an additional role for PECAM in the subsequent migration of monocytes through the subendothelial extracellular matrix. PECAM has six immunoglobulin (Ig) superfamily domains. Monoclonal antibodies whose epitopes map to domains 1 and/or 2 selectively block monocyte migration through the endothelial junction, whereas those that map to domain 6 block only the migration through the extracellular matrix, trapping the monocyte between the endothelium and its basal lamina. Therefore, transendothelial migration (diapedesis) and passage through extracellular matrix (interstitial migration) are distinct and separable phases of monocyte emigration. Furthermore, distinct and separate Ig domains of PECAM are involved in mediating these two steps.

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Selected References

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Funding 

Funders who supported this work.

NHLBI NIH HHS (1)

NIAID NIH HHS (1)