Interleukin (Il)-4 Is a Major Regulatory Cytokine Governing Bioactive IL-12 Production by Mouse and Human Dendritic Cells

doi:10.1084/jem.192.6.823

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J Exp Med. 2000 Sep 18; 192(6): 823–834.

doi: 10.1084/jem.192.6.823

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The effects of combinations of IL-4, GM-CSF, and IFN-γ on the IL-12 production by mouse splenic DCs. Mouse splenic DCs were cultured with a mix of stimuli as used in Fig. 1 in the presence or the absence of IL-4, IFN-γ, or GM-CSF, for 23 h. The supernatants were analyzed for IL-12 p70 and p40 by ELISA (A and B) and Western blotting (C). The result is typical of more than five similar experiments with ELISA readouts and Western blotting.

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