Endothelial expression of the Notch ligand Jagged1 is required for vascular smooth muscle development

High et al. 10.1073/pnas.0709663105.

Supporting Information

Files in this Data Supplement:

SI Figure 5
SI Table 1
SI Table 2




SI Figure 5

Fig. 5. Fate map of the Tie2-Cre mouse line. Tie2-Cre was crossed with a Cre-reporter mouse that expresses GFP upon Cre-mediated recombination. Section through the wall of the dorsal aorta of an E13.5 embryo, immunostained for GFP (green) and von Willebrand factor (vWF, red), showing Tie2-Cre-mediated recombination specifically in endothelial cells (arrowheads) and some hematopoietic cells (arrow).





Table 1. Genotypes of offspring obtained from Tie2-Cre +;Jag1flox/+ by Jag1flox/flox crosses

Genotype

Age

E8.5-E9.5

E10.5

E11.5

P0

Jag1flox/+

16

24

11

12

Jag1flox/flox

18

26

13

19

Tie2-Cre+;Jag1flox/+

17

20

12

13

Tie2-Cre+;Jag1flox/flox

11

29

12*

0

* All homozygous mutants at E11.5 were necrotic.





Table 2. Genotypes of offspring obtained from CMV-Cre+;Jag1flox/+ by Jag1flox/flox crosses

Genotype

Age

E8.5-E9.5

E10.5

E11.5

P0

Jag1flox/+

6

8

3

-

Jag1flox/flox

4

6

5

-

CMV-Cre+;Jag1flox/+

6

8

7

-

CMV-Cre+;Jag1flox/flox

6

9

6*

-

* All homozygous mutants at E11.5 were necrotic.