Europe PMC

This website requires cookies, and the limited processing of your personal data in order to function. By using the site you are agreeing to this as outlined in our privacy notice and cookie policy.

Cloning and expression of full-length cDNA encoding human vitamin D receptor.

Author information

Affiliations

  • 1. California Biotechnology Inc., Mountain View 94043.
      Authors
    • Baker AR 1
    (1 author)

ORCIDs linked to this article

Proceedings of the National Academy of Sciences of the United States of America, 01 May 1988, 85(10):3294-3298
https://doi.org/10.1073/pnas.85.10.3294 PMID: 2835767 PMCID: PMC280195

Free full text in Europe PMC

Abstract 

Complementary DNA clones encoding the human vitamin D receptor have been isolated from human intestine and T47D cell cDNA libraries. The nucleotide sequence of the 4605-base pair (bp) cDNA includes a noncoding leader sequence of 115 bp, a 1281-bp open reading frame, and 3209 bp of 3' noncoding sequence. Two polyadenylylation signals, AATAAA, are present 25 and 70 bp upstream of the poly(A) tail, respectively. RNA blot hybridization indicates a single mRNA species of approximately equal to 4600 bp. Transfection of the cloned sequences into COS-1 cells results in the production of a single receptor species indistinguishable from the native receptor. Sequence comparisons demonstrate that the vitamin D receptor belongs to the steroid-receptor gene family and is closest in size and sequence to another member of this family, the thyroid hormone receptor.

Free full text 

Logo of pnasLink to Publisher's site
Proc Natl Acad Sci U S A. 1988 May; 85(10): 3294–3298.
PMCID: PMC280195
PMID: 2835767

Cloning and expression of full-length cDNA encoding human vitamin D receptor.

A R Baker, D P McDonnell, M Hughes, T M Crisp, D J Mangelsdorf, M R Haussler, J W Pike, J Shine, and B W O'Malley
Author information Copyright and License information Disclaimer

Abstract

Complementary DNA clones encoding the human vitamin D receptor have been isolated from human intestine and T47D cell cDNA libraries. The nucleotide sequence of the 4605-base pair (bp) cDNA includes a noncoding leader sequence of 115 bp, a 1281-bp open reading frame, and 3209 bp of 3' noncoding sequence. Two polyadenylylation signals, AATAAA, are present 25 and 70 bp upstream of the poly(A) tail, respectively. RNA blot hybridization indicates a single mRNA species of approximately equal to 4600 bp. Transfection of the cloned sequences into COS-1 cells results in the production of a single receptor species indistinguishable from the native receptor. Sequence comparisons demonstrate that the vitamin D receptor belongs to the steroid-receptor gene family and is closest in size and sequence to another member of this family, the thyroid hormone receptor.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (1.0M), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.
 
icon of scanned page 3294
3294
icon of scanned page 3295
3295
icon of scanned page 3296
3296
icon of scanned page 3297
3297
icon of scanned page 3298
3298
 

Images in this article

Image
Image
on p.3295
Click on the image to see a larger version.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Haussler MR, McCain TA. Basic and clinical concepts related to vitamin D metabolism and action (second of two parts). N Engl J Med. 1977 Nov 10;297(19):1041–1050. [Abstract] [Google Scholar]
  • Pike JW. Intracellular receptors mediate the biologic action of 1,25-dihydroxyvitamin D3. Nutr Rev. 1985 Jun;43(6):161–168. [Abstract] [Google Scholar]
  • Haussler MR. Vitamin D receptors: nature and function. Annu Rev Nutr. 1986;6:527–562. [Abstract] [Google Scholar]
  • McDonnell DP, Mangelsdorf DJ, Pike JW, Haussler MR, O'Malley BW. Molecular cloning of complementary DNA encoding the avian receptor for vitamin D. Science. 1987 Mar 6;235(4793):1214–1217. [Abstract] [Google Scholar]
  • Hollenberg SM, Weinberger C, Ong ES, Cerelli G, Oro A, Lebo R, Thompson EB, Rosenfeld MG, Evans RM. Primary structure and expression of a functional human glucocorticoid receptor cDNA. Nature. 1985 Dec 19;318(6047):635–641. [Europe PMC free article] [Abstract] [Google Scholar]
  • Greene GL, Gilna P, Waterfield M, Baker A, Hort Y, Shine J. Sequence and expression of human estrogen receptor complementary DNA. Science. 1986 Mar 7;231(4742):1150–1154. [Abstract] [Google Scholar]
  • Green S, Walter P, Kumar V, Krust A, Bornert JM, Argos P, Chambon P. Human oestrogen receptor cDNA: sequence, expression and homology to v-erb-A. Nature. 1986 Mar 13;320(6058):134–139. [Abstract] [Google Scholar]
  • Weinberger C, Thompson CC, Ong ES, Lebo R, Gruol DJ, Evans RM. The c-erb-A gene encodes a thyroid hormone receptor. Nature. 1986 Dec 18;324(6098):641–646. [Abstract] [Google Scholar]
  • Misrahi M, Atger M, d'Auriol L, Loosfelt H, Meriel C, Fridlansky F, Guiochon-Mantel A, Galibert F, Milgrom E. Complete amino acid sequence of the human progesterone receptor deduced from cloned cDNA. Biochem Biophys Res Commun. 1987 Mar 13;143(2):740–748. [Abstract] [Google Scholar]
  • Denhardt DT. A membrane-filter technique for the detection of complementary DNA. Biochem Biophys Res Commun. 1966 Jun 13;23(5):641–646. [Abstract] [Google Scholar]
  • Rigby PW, Dieckmann M, Rhodes C, Berg P. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. J Mol Biol. 1977 Jun 15;113(1):237–251. [Abstract] [Google Scholar]
  • Okayama H, Berg P. High-efficiency cloning of full-length cDNA. Mol Cell Biol. 1982 Feb;2(2):161–170. [Europe PMC free article] [Abstract] [Google Scholar]
  • Sanger F, Nicklen S, Coulson AR. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. [Europe PMC free article] [Abstract] [Google Scholar]
  • Chirgwin JM, Przybyla AE, MacDonald RJ, Rutter WJ. Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease. Biochemistry. 1979 Nov 27;18(24):5294–5299. [Abstract] [Google Scholar]
  • Aviv H, Leder P. Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid-cellulose. Proc Natl Acad Sci U S A. 1972 Jun;69(6):1408–1412. [Europe PMC free article] [Abstract] [Google Scholar]
  • Wong GG, Witek JS, Temple PA, Wilkens KM, Leary AC, Luxenberg DP, Jones SS, Brown EL, Kay RM, Orr EC, et al. Human GM-CSF: molecular cloning of the complementary DNA and purification of the natural and recombinant proteins. Science. 1985 May 17;228(4701):810–815. [Abstract] [Google Scholar]
  • Deans RJ, Denis KA, Taylor A, Wall R. Expression of an immunoglobulin heavy chain gene transfected into lymphocytes. Proc Natl Acad Sci U S A. 1984 Mar;81(5):1292–1296. [Europe PMC free article] [Abstract] [Google Scholar]
  • Wecksler WR, Norman AW. An hydroxylapatite batch assay for the quantitation of 1alpha,25-dihydroxyvitamin D3-receptor complexes. Anal Biochem. 1979 Jan 15;92(2):314–323. [Abstract] [Google Scholar]
  • Mangelsdorf DJ, Pike JW, Haussler MR. Avian and mammalian receptors for 1,25-dihydroxyvitamin D3: in vitro translation to characterize size and hormone-dependent regulation. Proc Natl Acad Sci U S A. 1987 Jan;84(2):354–358. [Europe PMC free article] [Abstract] [Google Scholar]
  • Pike JW, Sleator NM. Hormone-dependent phosphorylation of the 1,25-dihydroxyvitamin D3 receptor in mouse fibroblasts. Biochem Biophys Res Commun. 1985 Aug 30;131(1):378–385. [Abstract] [Google Scholar]
  • Kozak M. Compilation and analysis of sequences upstream from the translational start site in eukaryotic mRNAs. Nucleic Acids Res. 1984 Jan 25;12(2):857–872. [Europe PMC free article] [Abstract] [Google Scholar]
  • Weinberger C, Hollenberg SM, Rosenfeld MG, Evans RM. Domain structure of human glucocorticoid receptor and its relationship to the v-erb-A oncogene product. Nature. 1985 Dec 19;318(6047):670–672. [Abstract] [Google Scholar]
  • Giguère V, Hollenberg SM, Rosenfeld MG, Evans RM. Functional domains of the human glucocorticoid receptor. Cell. 1986 Aug 29;46(5):645–652. [Abstract] [Google Scholar]
  • Miesfeld R, Rusconi S, Godowski PJ, Maler BA, Okret S, Wikström AC, Gustafsson JA, Yamamoto KR. Genetic complementation of a glucocorticoid receptor deficiency by expression of cloned receptor cDNA. Cell. 1986 Aug 1;46(3):389–399. [Abstract] [Google Scholar]
  • Kumar V, Green S, Staub A, Chambon P. Localisation of the oestradiol-binding and putative DNA-binding domains of the human oestrogen receptor. EMBO J. 1986 Sep;5(9):2231–2236. [Europe PMC free article] [Abstract] [Google Scholar]
  • Miller J, McLachlan AD, Klug A. Repetitive zinc-binding domains in the protein transcription factor IIIA from Xenopus oocytes. EMBO J. 1985 Jun;4(6):1609–1614. [Europe PMC free article] [Abstract] [Google Scholar]
  • Pike JW, Haussler MR. Association of 1,25-dihydroxyvitamin D3 with cultured 3T6 mouse fibroblasts. Cellular uptake and receptor-mediated migration to the nucleus. J Biol Chem. 1983 Jul 25;258(14):8554–8560. [Abstract] [Google Scholar]
  • Freake HC, Marcocci C, Iwasaki J, MacIntyre I. 1,25-dihydroxyvitamin D3 specifically binds to a human breast cancer cell line (T47D) and stimulates growth. Biochem Biophys Res Commun. 1981 Aug 31;101(4):1131–1138. [Abstract] [Google Scholar]
  • Putkey JA, Wecksler WR, Norman AW. The interaction of 1,25-dihydroxyvitamin D3 with its intestinal mucosa receptor: kinetic parameters and structural requirements. Lipids. 1978 Oct;13(10):723–729. [Abstract] [Google Scholar]
  • Mangelsdorf DJ, Koeffler HP, Donaldson CA, Pike JW, Haussler MR. 1,25-Dihydroxyvitamin D3-induced differentiation in a human promyelocytic leukemia cell line (HL-60): receptor-mediated maturation to macrophage-like cells. J Cell Biol. 1984 Feb;98(2):391–398. [Europe PMC free article] [Abstract] [Google Scholar]
  • Yamamoto KR. Steroid receptor regulated transcription of specific genes and gene networks. Annu Rev Genet. 1985;19:209–252. [Abstract] [Google Scholar]
  • Marx SJ, Liberman UA, Eil C, Gamblin GT, DeGrange DA, Balsan S. Hereditary resistance to 1,25-dihydroxyvitamin D. Recent Prog Horm Res. 1984;40:589–620. [Abstract] [Google Scholar]
Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

Full text links 

Read article at publisher's site: https://doi.org/10.1073/pnas.85.10.3294

Read article for free, from open access legal sources, via Unpaywall: http://www.pnas.org/content/85/10/3294.full.pdfUnpaywall

Citations & impact 

Impact metrics

434
Citations
Jump to Citations
6
Data citations
Jump to Data

Citations of article over time

Alternative metrics

Altmetric item for https://www.altmetric.com/details/3374911
Altmetric
Discover the attention surrounding your research
https://www.altmetric.com/details/3374911

Article citations

Go to all (434) article citations

Other citations

Data 

Similar Articles 

To arrive at the top five similar articles we use a word-weighted algorithm to compare words from the Title and Abstract of each citation.