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Attaching and effacing locus of a Citrobacter freundii biotype that causes transmissible murine colonic hyperplasia.

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  • 1. Department of Microbiology and Immunology, Stanford University School of Medicine, California 94305-5402.
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    • Schauer DB 1
    (1 author)

Infection and Immunity, 01 Jun 1993, 61(6):2486-2492
https://doi.org/10.1128/iai.61.6.2486-2492.1993 PMID: 8500884 PMCID: PMC280873

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Abstract 

Citrobacter freundii biotype 4280 produces attaching and effacing (AE) lesions in the large intestine of laboratory mice and is the causative agent of transmissible murine colonic hyperplasia. AE lesions are also produced by enteropathogenic Escherichia coli in humans. Southern analysis revealed that biotype 4280, but not 20 other strains of C. freundii, contained DNA homologous to the eae (E. coli attaching and effacing) gene which is necessary for AE activity by enteropathogenic E. coli in vitro. We have cloned and determined the nucleotide sequence of the C. freundii eae homolog. Our findings suggest that the eae locus of C. freundii biotype 4280 is necessary for AE activity and has a role in the pathogenesis of transmissible murine colonic hyperplasia.

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Infect Immun. 1993 Jun; 61(6): 2486–2492.
PMCID: PMC280873
PMID: 8500884

Attaching and effacing locus of a Citrobacter freundii biotype that causes transmissible murine colonic hyperplasia.

D B Schauer and S Falkow
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Abstract

Citrobacter freundii biotype 4280 produces attaching and effacing (AE) lesions in the large intestine of laboratory mice and is the causative agent of transmissible murine colonic hyperplasia. AE lesions are also produced by enteropathogenic Escherichia coli in humans. Southern analysis revealed that biotype 4280, but not 20 other strains of C. freundii, contained DNA homologous to the eae (E. coli attaching and effacing) gene which is necessary for AE activity by enteropathogenic E. coli in vitro. We have cloned and determined the nucleotide sequence of the C. freundii eae homolog. Our findings suggest that the eae locus of C. freundii biotype 4280 is necessary for AE activity and has a role in the pathogenesis of transmissible murine colonic hyperplasia.

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Selected References

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