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Lifeact: a versatile marker to visualize F-actin.

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Affiliations

  • 1. Max Planck Institute of Biochemistry, Independent Junior Research Group Cellular Dynamics and Cell Patterning, Am Klopferspitz 18, 82152 Martinsried, Germany.
      Authors
    • Riedl J 1
    (1 author)

ORCIDs linked to this article

Nature Methods, 08 Jun 2008, 5(7):605-607
https://doi.org/10.1038/nmeth.1220 PMID: 18536722 PMCID: PMC2814344

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A comment on this article appears in "Lifeact cannot visualize some forms of stress-induced twisted F-actin." Nat Methods. 2009 May;6(5):317.

Abstract 

Live imaging of the actin cytoskeleton is crucial for the study of many fundamental biological processes, but current approaches to visualize actin have several limitations. Here we describe Lifeact, a 17-amino-acid peptide, which stained filamentous actin (F-actin) structures in eukaryotic cells and tissues. Lifeact did not interfere with actin dynamics in vitro and in vivo and in its chemically modified peptide form allowed visualization of actin dynamics in nontransfectable cells.

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Read article at publisher's site: https://doi.org/10.1038/nmeth.1220

Read article for free, from open access legal sources, via Unpaywall: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2814344Unpaywall

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Funding 

Funders who supported this work.

NIAID NIH HHS (2)