Figure 7.

Liver MDSC inhibit CTL-mediated cytolysis. Splenocytes from mice twice immunized with DC.Ova were harvested 1 week after the second immunization and restimulated in vitro with Ova for 5 days before cytolysis assay using EG7 targets. In parallel, hepatic CD11b⁺Gr1⁺ cells from i.p. MCA38-bearing hosts were mixed with the restimulated splenocytes in a 1:1 ratio before assay. MDSC eliminated CTL lysis (P<0.05 for all E:T ratios). Experiments were done in triplicate using three mice/group and repeated twice with similar results. *, P < 0.05.