Logo of jcinvestThe Journal of Clinical Investigation
PMC full text:
Copyright/LicenseRequest permission to reuse

Figure 10

An external file that holds a picture, illustration, etc. Object name is JCI43004.f10.jpg
Oxidation-mediated multimerization of short and long sFasL.

Western blot analyses of short and long rh-sFasL incubated in PBS alone or in the presence of 2 different concentrations of HOCl (μM). After 15 minutes, the reaction was terminated with 2.5 nM l-methionine. Both short and long human sFasL have the same aggregation status in basal conditions. Following oxidation with HOCl, short and long rh-sFasL showed similar multimerization (50-kDa and larger molecular weight bands). Western blot analyses were performed using SDS-PAGE with reducing conditions (2-ME and heat).

Images in this article

  • Figure 1
  • Figure 2
  • Figure 3
  • Figure 4
  • Figure 5
  • Figure 6
  • Figure 7
  • Figure 8
  • Figure 9
  • Figure 10
  • Figure 11
  • Figure 12
  • Figure 13
  • Figure 14
Click on the image to see a larger version.