Expression of fhaBBb in B. pertussis does not alter its ability to infect miceA. The number of cfu recovered from the lungs of BALB/c mice at days 0, 3 or 4, and 11 post inoculation with 50 μl PBS containing 5 × 105 cfu of the indicated strains is shown. Each circle represents the number of cfu recovered from a single animal. The horizontal black bar is the geometric mean for each group. For RBX20 at day 3/4, the means of numbers from moribund animals (~109) and healthy animals (~105) were calculated separately as shown. The dashed line represents the lower limit of detection. Asterisks indicate a statistically significant difference compared with RB50 (P < 0.05). This experiment has been performed at least twice with similar results. The data from one experiment are shown.
B. Western blots of whole cell lysates of the strains indicated across the bottom probed with serum from mice inoculated with PBS or the strains indicated across the top. The sera were collected 21 days post inoculation. Molecular mass markers are shown on the right.