Europe PMC

This website requires cookies, and the limited processing of your personal data in order to function. By using the site you are agreeing to this as outlined in our privacy notice and cookie policy.

Colony-stimulating factor 1 expression is down-regulated during the adipocyte differentiation of H-1/A marrow stromal cells and induced by cachectin/tumor necrosis factor.

Author information

Affiliations

  • 1. Department of Pathology, Keio University School of Medicine, Tokyo, Japan.
      Authors
    • Umezawa A 1
    (1 author)

Molecular and Cellular Biology, 01 Feb 1991, 11(2):920-927
https://doi.org/10.1128/mcb.11.2.920-927.1991 PMID: 1990292 PMCID: PMC359750

Free full text in Europe PMC

Abstract 

We isolated clonal sublines of the established mouse marrow stromal cell line, H-1. These clonal sublines underwent differentiation into adipocytes in various degrees. One subline, H-1/A, underwent adipocyte differentiation after confluence, while another subline, H-1/D, did not differentiate. In H-1/A cells, the 4.5- and 2.5-kb major mRNA species of colony-stimulating factor 1 (CSF-1) were expressed before differentiation and were down-regulated at a posttranscriptional level during the differentiation of H-1/A cells. The down-regulation of the CSF-1 gene was not a result of arrested cellular growth, because no down-regulation was detected in the nondifferentiating sister line, H-1/D. This down-regulation appeared to be an early event in differentiation. Cachectin/tumor necrosis factor transiently induced the expression of CSF-1 and inhibited the differentiation of H-1/A cells into adipocytes. This induced expression of CSF-1 was due to an increased rate of transcription.

Free full text 

Logo of molcellbLink to Publisher's site
Mol Cell Biol. 1991 Feb; 11(2): 920–927.
PMCID: PMC359750
PMID: 1990292

Colony-stimulating factor 1 expression is down-regulated during the adipocyte differentiation of H-1/A marrow stromal cells and induced by cachectin/tumor necrosis factor.

A Umezawa, K Tachibana, K Harigaya, S Kusakari, S Kato, Y Watanabe, and T Takano
Author information Copyright and License information Disclaimer

Abstract

We isolated clonal sublines of the established mouse marrow stromal cell line, H-1. These clonal sublines underwent differentiation into adipocytes in various degrees. One subline, H-1/A, underwent adipocyte differentiation after confluence, while another subline, H-1/D, did not differentiate. In H-1/A cells, the 4.5- and 2.5-kb major mRNA species of colony-stimulating factor 1 (CSF-1) were expressed before differentiation and were down-regulated at a posttranscriptional level during the differentiation of H-1/A cells. The down-regulation of the CSF-1 gene was not a result of arrested cellular growth, because no down-regulation was detected in the nondifferentiating sister line, H-1/D. This down-regulation appeared to be an early event in differentiation. Cachectin/tumor necrosis factor transiently induced the expression of CSF-1 and inhibited the differentiation of H-1/A cells into adipocytes. This induced expression of CSF-1 was due to an increased rate of transcription.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (2.0M), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.
 
icon of scanned page 920
920
icon of scanned page 921
921
icon of scanned page 922
922
icon of scanned page 923
923
icon of scanned page 924
924
icon of scanned page 925
925
icon of scanned page 926
926
icon of scanned page 927
927
 

Images in this article

Image
Image
on p.922
Image
Image
on p.922
Image
Image
on p.923
Image
Image
on p.924
Image
Image
on p.925
Click on the image to see a larger version.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Bastos RN, Volloch Z, Aviv H. Messenger RNA population analysis during erythroid differentiation: a kinetical approach. J Mol Biol. 1977 Feb 25;110(2):191–203. [Abstract] [Google Scholar]
  • Bories D, Raynal MC, Solomon DH, Darzynkiewicz Z, Cayre YE. Down-regulation of a serine protease, myeloblastin, causes growth arrest and differentiation of promyelocytic leukemia cells. Cell. 1989 Dec 22;59(6):959–968. [Abstract] [Google Scholar]
  • Broudy VC, Kaushansky K, Segal GM, Harlan JM, Adamson JW. Tumor necrosis factor type alpha stimulates human endothelial cells to produce granulocyte/macrophage colony-stimulating factor. Proc Natl Acad Sci U S A. 1986 Oct;83(19):7467–7471. [Europe PMC free article] [Abstract] [Google Scholar]
  • Chirgwin JM, Przybyla AE, MacDonald RJ, Rutter WJ. Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease. Biochemistry. 1979 Nov 27;18(24):5294–5299. [Abstract] [Google Scholar]
  • Chung S, Landfear SM, Blumberg DD, Cohen NS, Lodish HF. Synthesis and stability of developmentally regulated dictyostelium mRNAs are affected by cell--cell contact and cAMP. Cell. 1981 Jun;24(3):785–797. [Abstract] [Google Scholar]
  • Coppola JA, Cole MD. Constitutive c-myc oncogene expression blocks mouse erythroleukaemia cell differentiation but not commitment. Nature. 1986 Apr 24;320(6064):760–763. [Abstract] [Google Scholar]
  • Darnell JE., Jr Variety in the level of gene control in eukaryotic cells. Nature. 1982 Jun 3;297(5865):365–371. [Abstract] [Google Scholar]
  • Feinberg AP, Vogelstein B. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal Biochem. 1983 Jul 1;132(1):6–13. [Abstract] [Google Scholar]
  • Fung MC, Hapel AJ, Ymer S, Cohen DR, Johnson RM, Campbell HD, Young IG. Molecular cloning of cDNA for murine interleukin-3. Nature. 1984 Jan 19;307(5948):233–237. [Abstract] [Google Scholar]
  • Gimble JM, Dorheim MA, Cheng Q, Medina K, Wang CS, Jones R, Koren E, Pietrangeli C, Kincade PW. Adipogenesis in a murine bone marrow stromal cell line capable of supporting B lineage lymphocyte growth and proliferation: biochemical and molecular characterization. Eur J Immunol. 1990 Feb;20(2):379–387. [Abstract] [Google Scholar]
  • Groudine M, Peretz M, Weintraub H. Transcriptional regulation of hemoglobin switching in chicken embryos. Mol Cell Biol. 1981 Mar;1(3):281–288. [Europe PMC free article] [Abstract] [Google Scholar]
  • Gough NM, Gough J, Metcalf D, Kelso A, Grail D, Nicola NA, Burgess AW, Dunn AR. Molecular cloning of cDNA encoding a murine haematopoietic growth regulator, granulocyte-macrophage colony stimulating factor. Nature. 309(5971):763–767. [Abstract] [Google Scholar]
  • Green H, Meuth M. An established pre-adipose cell line and its differentiation in culture. Cell. 1974 Oct;3(2):127–133. [Abstract] [Google Scholar]
  • Guilbert LJ, Stanley ER. Specific interaction of murine colony-stimulating factor with mononuclear phagocytic cells. J Cell Biol. 1980 Apr;85(1):153–159. [Europe PMC free article] [Abstract] [Google Scholar]
  • Harigaya K, Cronkite EP, Miller ME, Shadduck RK. Murine bone marrow cell line producing colony-stimulating factor. Proc Natl Acad Sci U S A. 1981 Nov;78(11):6963–6966. [Europe PMC free article] [Abstract] [Google Scholar]
  • Harigaya K, Todoroki T, Nakamura M, Watanabe Y. Conversion to adipocytes of a clonal bone marrow preadipocyte line (H-1/A) and fatty acid composition of the resultant adipocytes. Proc Soc Exp Biol Med. 1986 Apr;181(4):523–528. [Abstract] [Google Scholar]
  • Horiguchi J, Sariban E, Kufe D. Transcriptional and posttranscriptional regulation of CSF-1 gene expression in human monocytes. Mol Cell Biol. 1988 Sep;8(9):3951–3954. [Europe PMC free article] [Abstract] [Google Scholar]
  • Horiguchi J, Warren MK, Ralph P, Kufe D. Expression of the macrophage specific colony-stimulating factor (CSF-1) during human monocytic differentiation. Biochem Biophys Res Commun. 1986 Dec 30;141(3):924–930. [Abstract] [Google Scholar]
  • Hu MC, Sharp SB, Davidson N. The complete sequence of the mouse skeletal alpha-actin gene reveals several conserved and inverted repeat sequences outside of the protein-coding region. Mol Cell Biol. 1986 Jan;6(1):15–25. [Europe PMC free article] [Abstract] [Google Scholar]
  • Hunter T, Sefton BM. Transforming gene product of Rous sarcoma virus phosphorylates tyrosine. Proc Natl Acad Sci U S A. 1980 Mar;77(3):1311–1315. [Europe PMC free article] [Abstract] [Google Scholar]
  • Kawasaki ES, Ladner MB, Wang AM, Van Arsdell J, Warren MK, Coyne MY, Schweickart VL, Lee MT, Wilson KJ, Boosman A, et al. Molecular cloning of a complementary DNA encoding human macrophage-specific colony-stimulating factor (CSF-1). Science. 1985 Oct 18;230(4723):291–296. [Abstract] [Google Scholar]
  • Koeffler HP, Gasson J, Ranyard J, Souza L, Shepard M, Munker R. Recombinant human TNF alpha stimulates production of granulocyte colony-stimulating factor. Blood. 1987 Jul;70(1):55–59. [Abstract] [Google Scholar]
  • Lachman HM, Skoultchi AI. Expression of c-myc changes during differentiation of mouse erythroleukaemia cells. Nature. 1984 Aug 16;310(5978):592–594. [Abstract] [Google Scholar]
  • Ladner MB, Martin GA, Noble JA, Nikoloff DM, Tal R, Kawasaki ES, White TJ. Human CSF-1: gene structure and alternative splicing of mRNA precursors. EMBO J. 1987 Sep;6(9):2693–2698. [Europe PMC free article] [Abstract] [Google Scholar]
  • Ladner MB, Martin GA, Noble JA, Wittman VP, Warren MK, McGrogan M, Stanley ER. cDNA cloning and expression of murine macrophage colony-stimulating factor from L929 cells. Proc Natl Acad Sci U S A. 1988 Sep;85(18):6706–6710. [Europe PMC free article] [Abstract] [Google Scholar]
  • Lomedico PT, Gubler U, Hellmann CP, Dukovich M, Giri JG, Pan YC, Collier K, Semionow R, Chua AO, Mizel SB. Cloning and expression of murine interleukin-1 cDNA in Escherichia coli. Nature. 312(5993):458–462. [Abstract] [Google Scholar]
  • Li CY, Lam KW, Yam LT. Esterases in human leukocytes. J Histochem Cytochem. 1973 Jan;21(1):1–12. [Abstract] [Google Scholar]
  • Morrison DK, Browning PJ, White MF, Roberts TM. Tyrosine phosphorylations in vivo associated with v-fms transformation. Mol Cell Biol. 1988 Jan;8(1):176–185. [Europe PMC free article] [Abstract] [Google Scholar]
  • Munker R, Gasson J, Ogawa M, Koeffler HP. Recombinant human TNF induces production of granulocyte-monocyte colony-stimulating factor. Nature. 1986 Sep 4;323(6083):79–82. [Abstract] [Google Scholar]
  • Nakamura M, Harigaya K, Watanabe Y. Correlation between production of colony-stimulating activity (CSA) and adipose conversion in a murine marrow-derived preadipocyte line (H-1/A). Proc Soc Exp Biol Med. 1985 Jul;179(3):283–287. [Abstract] [Google Scholar]
  • Oliff A, Defeo-Jones D, Boyer M, Martinez D, Kiefer D, Vuocolo G, Wolfe A, Socher SH. Tumors secreting human TNF/cachectin induce cachexia in mice. Cell. 1987 Aug 14;50(4):555–563. [Abstract] [Google Scholar]
  • Oster W, Lindemann A, Horn S, Mertelsmann R, Herrmann F. Tumor necrosis factor (TNF)-alpha but not TNF-beta induces secretion of colony stimulating factor for macrophages (CSF-1) by human monocytes. Blood. 1987 Nov;70(5):1700–1703. [Abstract] [Google Scholar]
  • Pike BL, Robinson WA. Human bone marrow colony growth in agar-gel. J Cell Physiol. 1970 Aug;76(1):77–84. [Abstract] [Google Scholar]
  • Rajavashisth TB, Eng R, Shadduck RK, Waheed A, Ben-Avram CM, Shively JE, Lusis AJ. Cloning and tissue-specific expression of mouse macrophage colony-stimulating factor mRNA. Proc Natl Acad Sci U S A. 1987 Mar;84(5):1157–1161. [Europe PMC free article] [Abstract] [Google Scholar]
  • Rambaldi A, Wakamiya N, Vellenga E, Horiguchi J, Warren MK, Kufe D, Griffin JD. Expression of the macrophage colony-stimulating factor and c-fms genes in human acute myeloblastic leukemia cells. J Clin Invest. 1988 Apr;81(4):1030–1035. [Europe PMC free article] [Abstract] [Google Scholar]
  • Rambaldi A, Young DC, Griffin JD. Expression of the M-CSF (CSF-1) gene by human monocytes. Blood. 1987 May;69(5):1409–1413. [Abstract] [Google Scholar]
  • Rettenmier CW, Roussel MF, Ashmun RA, Ralph P, Price K, Sherr CJ. Synthesis of membrane-bound colony-stimulating factor 1 (CSF-1) and downmodulation of CSF-1 receptors in NIH 3T3 cells transformed by cotransfection of the human CSF-1 and c-fms (CSF-1 receptor) genes. Mol Cell Biol. 1987 Jul;7(7):2378–2387. [Europe PMC free article] [Abstract] [Google Scholar]
  • Roussel MF, Dull TJ, Rettenmier CW, Ralph P, Ullrich A, Sherr CJ. Transforming potential of the c-fms proto-oncogene (CSF-1 receptor). Nature. 1987 Feb 5;325(6104):549–552. [Abstract] [Google Scholar]
  • Roussel MF, Rettenmier CW, Look AT, Sherr CJ. Cell surface expression of v-fms-coded glycoproteins is required for transformation. Mol Cell Biol. 1984 Oct;4(10):1999–2009. [Europe PMC free article] [Abstract] [Google Scholar]
  • Sherr CJ, Rettenmier CW, Sacca R, Roussel MF, Look AT, Stanley ER. The c-fms proto-oncogene product is related to the receptor for the mononuclear phagocyte growth factor, CSF-1. Cell. 1985 Jul;41(3):665–676. [Abstract] [Google Scholar]
  • Sherman ML, Weber BL, Datta R, Kufe DW. Transcriptional and posttranscriptional regulation of macrophage-specific colony stimulating factor gene expression by tumor necrosis factor. Involvement of arachidonic acid metabolites. J Clin Invest. 1990 Feb;85(2):442–447. [Europe PMC free article] [Abstract] [Google Scholar]
  • Sherry B, Cerami A. Cachectin/tumor necrosis factor exerts endocrine, paracrine, and autocrine control of inflammatory responses. J Cell Biol. 1988 Oct;107(4):1269–1277. [Europe PMC free article] [Abstract] [Google Scholar]
  • Spiegelman BM, Farmer SR. Decreases in tubulin and actin gene expression prior to morphological differentiation of 3T3 adipocytes. Cell. 1982 May;29(1):53–60. [Abstract] [Google Scholar]
  • Stanley ER, Chen DM, Lin HS. Induction of macrophage production and proliferation by a purified colony stimulating factor. Nature. 1978 Jul 13;274(5667):168–170. [Abstract] [Google Scholar]
  • Stanley ER, Guilbert LJ, Tushinski RJ, Bartelmez SH. CSF-1--a mononuclear phagocyte lineage-specific hemopoietic growth factor. J Cell Biochem. 1983;21(2):151–159. [Abstract] [Google Scholar]
  • Torti FM, Dieckmann B, Beutler B, Cerami A, Ringold GM. A macrophage factor inhibits adipocyte gene expression: an in vitro model of cachexia. Science. 1985 Aug 30;229(4716):867–869. [Abstract] [Google Scholar]
  • Tsuchiya M, Asano S, Kaziro Y, Nagata S. Isolation and characterization of the cDNA for murine granulocyte colony-stimulating factor. Proc Natl Acad Sci U S A. 1986 Oct;83(20):7633–7637. [Europe PMC free article] [Abstract] [Google Scholar]
  • Umezawa A, Harigaya K, Abe H, Watanabe Y. Gap-junctional communication of bone marrow stromal cells is resistant to irradiation in vitro. Exp Hematol. 1990 Oct;18(9):1002–1007. [Abstract] [Google Scholar]
Articles from Molecular and Cellular Biology are provided here courtesy of Taylor & Francis

Full text links 

Read article at publisher's site: https://doi.org/10.1128/mcb.11.2.920-927.1991

Read article for free, from open access legal sources, via Unpaywall: https://europepmc.org/articles/pmc359750?pdf=renderUnpaywall

Free after 4 months at mcb.asm.org
http://mcb.asm.org/cgi/reprint/11/2/920

Free to read at mcb.asm.org
http://mcb.asm.org/cgi/content/abstract/11/2/920

Citations & impact 

Impact metrics

34
Citations
Jump to Citations

Citations of article over time

Article citations

Go to all (34) article citations

Similar Articles 

To arrive at the top five similar articles we use a word-weighted algorithm to compare words from the Title and Abstract of each citation.