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Fig. 4.

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CCDC41 and IFT20 interact with each other for proper localization. (A) RPE cells were transfected with FLAG-IFT20 plasmid and doubled-labeled with anti-FLAG and anti-CCDC41 antibodies. (B) HEK293T cells were transfected with the indicated plasmid vectors for 16 h. Cell lysates were immunoprecipitated with anti-FLAG antibody conjugated with agarose beads, and the resulting precipitates were immunoblotted with anti-GFP antibody. (C) RPE1 cells were transfected with siRNAs for 3 d (cells were serum-starved for the final 24 h), and double-stained with anti-CCDC41 and anti–glu-Tub antibodies. (D) RPE1 cells were transfected with siRNAs for 3 d (cells were serum-starved for the final 24 h), and double-stained with anti-IFT20 and anti–glu-Tub antibodies. (Scale bars: A, 10 μm; C and D, 20 μm.)

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