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Isolation of antibodies for phosphotyrosine by immunization with a v-abl oncogene-encoded protein.

Molecular and Cellular Biology, 01 Dec 1985, 5(12):3640-3643
https://doi.org/10.1128/mcb.5.12.3640-3643.1985 PMID: 2427929 PMCID: PMC369198

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Abstract 

Immunization of rabbits with a tyrosine-phosphorylated v-abl protein resulted in the production of antibodies for the v-abl protein and for phosphotyrosine. The antiphosphotyrosine antibodies could be purified by affinity chromatography with O-phosphotyramine coupled to Sepharose. These antibodies detected a variety of tyrosine-phosphorylated proteins, including receptors for peptide growth factors. The usefulness of these antibodies was demonstrated by the detection of previously unidentified tyrosine-phosphorylated proteins in v-src-, v-abl-, and v-erbB-transformed cell lines.

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Mol Cell Biol. 1985 Dec; 5(12): 3640–3643.
PMCID: PMC369198
PMID: 2427929

Isolation of antibodies for phosphotyrosine by immunization with a v-abl oncogene-encoded protein.

J Y Wang
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Abstract

Immunization of rabbits with a tyrosine-phosphorylated v-abl protein resulted in the production of antibodies for the v-abl protein and for phosphotyrosine. The antiphosphotyrosine antibodies could be purified by affinity chromatography with O-phosphotyramine coupled to Sepharose. These antibodies detected a variety of tyrosine-phosphorylated proteins, including receptors for peptide growth factors. The usefulness of these antibodies was demonstrated by the detection of previously unidentified tyrosine-phosphorylated proteins in v-src-, v-abl-, and v-erbB-transformed cell lines.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.
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