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Identification and quantitation of platelet-associated fibronectin antigen.

The Journal of Clinical Investigation, 01 Mar 1979, 63(3):540-543
https://doi.org/10.1172/jci109334 PMID: 372243 PMCID: PMC371985

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Abstract 

Platelet-associated fibronectin antigen has been identified by radioimmunoassay and immunofluorescent techniques. In radioimmunoassay, platelet fibronectin was immunochemically indistinguishable from plasma fibronectin. Platelet and plasma fibronectin were bound and eluted from gelatin-sepharose under similar conditions. The level of platelet fibronectin in detergent extracts of washed platelets from 12 healthy adults was 2.85 +/- 1.24 microgram/10(9) platelets. Immunofluorescence with F(ab')2 fragments of immunochemically purified antifibronectin showed that all platelets stained with a discrete punctate pattern. The identification of platelet fibronectin antigen raises the possibility that this protein may participate in platelet-platelet or platelet-surface interactions.

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Logo of jcinvestThe Journal of Clinical Investigation
J Clin Invest. 1979 Mar; 63(3): 540–543.
PMCID: PMC371985
PMID: 372243

Identification and quantitation of platelet-associated fibronectin antigen.

E F Plow, C Birdwell, and M H Ginsberg
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Abstract

Platelet-associated fibronectin antigen has been identified by radioimmunoassay and immunofluorescent techniques. In radioimmunoassay, platelet fibronectin was immunochemically indistinguishable from plasma fibronectin. Platelet and plasma fibronectin were bound and eluted from gelatin-sepharose under similar conditions. The level of platelet fibronectin in detergent extracts of washed platelets from 12 healthy adults was 2.85 +/- 1.24 microgram/10(9) platelets. Immunofluorescence with F(ab')2 fragments of immunochemically purified antifibronectin showed that all platelets stained with a discrete punctate pattern. The identification of platelet fibronectin antigen raises the possibility that this protein may participate in platelet-platelet or platelet-surface interactions.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.
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