Correction: Exponential Megapriming PCR (EMP) Cloning—Seamless DNA Insertion into Any Target Plasmid without Sequence Constraints
Free full text in Europe PMC
Abstract
No abstract provided.
Free full text
PLoS One. 2013; 8(9): 10.1371/annotation/7465cec8-d898-4d11-b32d-fcd1a8c24f25.
Published online 2013 Sep 20. https://doi.org/10.1371/annotation/7465cec8-d898-4d11-b32d-fcd1a8c24f25
PMCID: PMC3779282
Correction: Exponential Megapriming PCR (EMP) Cloning—Seamless DNA Insertion into Any Target Plasmid without Sequence Constraints
This corrects the article "Exponential Megapriming PCR (EMP) Cloning—Seamless DNA Insertion into Any Target Plasmid without Sequence Constraints" in volume 7, e53360.
An error was introduced in the preparation of this article for publication. The error is in the penultimate sentence of the "One-step EMP Cloning" section of the Materials and Methods.
The correct sentence is: A typical reaction contains 0.02 μM primer R1, 0,5 μM primer F1 and 0.5 μM primer R2 in addition to 1x HF Phusion buffer, 200 μM of each dNTP, 25 ng template DNA for the insert, 25 ng template DNA for the target plasmid, and 0.02 U/μL Phusion DNA Polymerase (NEB) in a 50 μL reaction.
Footnotes
Competing Interests: No competing interests declared.
Articles from PLOS ONE are provided here courtesy of PLOS
