Figure 7

CD115⁺GFP⁺ blood monocyte subsets Ly-6C⁺MHCII⁺, Ly-6C⁺MHCII⁻, and Ly-6C⁻MHCII⁻ were sorted from CD45.2 Cx3cr1^gfp/gfp mice. Sorted monocytes 1×10⁶ Ly-6C⁺MHCII⁻, 3×10⁵ Ly-6C⁺MHCII⁺ or 1×10⁶ Ly-6C⁻MHCII⁻ were injected i.v. into CD45.1⁺ WT mice, followed by i.d. injection of 2 μg LPS. At 18 h, (A) blood, (B) skin-draining LNs (SLN), and (C) skin were analyzed for monocytes migration and differentiation. Dot plot overlays display total CD11b⁺ cells (gray) and CD45.2 GFP⁺ adoptively transferred cells (black). C) Skin illustrates two separate experiments (exp 1, exp 2) adoptively transferring Ly-6C⁺MHCII⁻ monocytes. D) Purified Ly-6C⁺MHCII⁻ and Ly-6C⁺MHCII⁺ blood monocytes were injected intradermally into skin and recovered 18 h later to assess MHC II expression. E) Splenic Ly-6C⁺MHCII⁻ monocytes, depleted completely of MHC II⁺ cells, were enriched by negative selection and cocultured with various cell types as shown. Ly-6C⁺ monocytes were gated before and after the cocultures and the induction of surface MHC II was assessed and plotted (on the right). Each data point represents one experiment.