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Utilization of the beta and gamma chains of the IL-2 receptor by the novel cytokine IL-15.

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Affiliations

  • 1. Immunex Research and Development Corporation, Seattle, WA 98101.
      Authors
    • Giri JG 1
    (1 author)

The EMBO Journal, 01 Jun 1994, 13(12):2822-2830
https://doi.org/10.1002/j.1460-2075.1994.tb06576.x PMID: 8026467 PMCID: PMC395163

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Abstract 

We have recently cloned a novel cytokine, IL-15, with shared bioactivities but no sequence homology with IL-2. We found high affinity IL-15 binding to many cell types, including cells of non-lymphoid origin. Analysis of IL-15 interaction with subunits of the IL-2 receptor (IL-2R) revealed that the alpha subunit was not involved in IL-15 binding. We demonstrated directly in cells transfected with IL-2R subunits that both the beta and gamma chains are required for IL-15 binding and signaling. Hence, IL-15, like IL-2, IL-4 and IL-7, utilizes the common IL-2R gamma subunit found to be defective in X-linked severe combined immunodeficiency in humans. IL-15 is the only cytokine other than IL-2 that has also been shown to share the beta signaling subunit of IL-2R. The differential ability of some cells to bind and respond to IL-2 and IL-15 implies the existence of an additional IL-15-specific component.

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Logo of embojLink to Publisher's site
EMBO J. 1994 Jun 15; 13(12): 2822–2830.
PMCID: PMC395163
PMID: 8026467

Utilization of the beta and gamma chains of the IL-2 receptor by the novel cytokine IL-15.

J G Giri, M Ahdieh, J Eisenman, K Shanebeck, K Grabstein, S Kumaki, A Namen, L S Park, D Cosman, and D Anderson
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Abstract

We have recently cloned a novel cytokine, IL-15, with shared bioactivities but no sequence homology with IL-2. We found high affinity IL-15 binding to many cell types, including cells of non-lymphoid origin. Analysis of IL-15 interaction with subunits of the IL-2 receptor (IL-2R) revealed that the alpha subunit was not involved in IL-15 binding. We demonstrated directly in cells transfected with IL-2R subunits that both the beta and gamma chains are required for IL-15 binding and signaling. Hence, IL-15, like IL-2, IL-4 and IL-7, utilizes the common IL-2R gamma subunit found to be defective in X-linked severe combined immunodeficiency in humans. IL-15 is the only cytokine other than IL-2 that has also been shown to share the beta signaling subunit of IL-2R. The differential ability of some cells to bind and respond to IL-2 and IL-15 implies the existence of an additional IL-15-specific component.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.
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