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FIG. 3.

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Western blot to detect possible interaction between Tal2009-Δ5N and Tal2009ΔC. The negative control is in lane 2, and the lysates of E. coli cultures overexpressing protein from pTal2009ΔC and pTal2009-5ΔNtet are in lanes 3 and 4, respectively. Lysates resulting from concomitant induction of expression from pTal2009-5ΔNtet and pTal2009ΔC can be observed in lane 5, with a band of ~30 kDa denoted by an arrow. Lane 1 was loaded with a six-His protein ladder which reacts to the commercial Abs, and the molecular masses are indicated.

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