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Published online 2015 Jan 13. Prepublished online 2014 Nov 21. doi: 10.1128/AEM.03695-14
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FIG 3

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Transmission electron micrographs of the purified fusion proteins. (A) TEM analysis of NvC-aD. (B) NvC-aD probed with anti-HBsAg, followed by protein A conjugated to 5-nm gold particles. The arrows indicate gold particles. (C) NvC probed with anti-HBsAg, followed by protein A conjugated to 5-nm gold particles. The samples were stained negatively with uranyl acetate (1% [wt/vol]) and viewed under ×100,000 magnification.

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