Group Selection and Contribution of Minority Variants during Virus Adaptation Determines Virus Fitness and Phenotype

doi:10.1371/journal.ppat.1004838

PMC full text:

PLoS Pathog. 2015 May; 11(5): e1004838.

Published online 2015 May 5. doi: 10.1371/journal.ppat.1004838

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Table 1

Minority variants (>1% threshold) present in the passage 40 populations of the six CVB3 replicates, passaged in either HeLa or A549 cells.

		replicate						receptor
	capsid protein	1	2	3	4	5	6	footprint
HeLa	VP1	K259M						CAR
				K259N	K259N
		K259R		K259R	K259R	K259R	K259R
	VP2	D138E	D138E	D138E	D138E	D138E	D138E	CAR/DAF
		D138G	D138G			D138G	D138G
		D138N	D138N	D138N	D138N
	VP3			N63H	N63H		N63H	DAF
		Q234K	Q234K
						Q234R	Q234R
A549	VP1	T271P	T271P					DAF
	VP2	D138E		D138E	D138E	D138E	D138E	CAR/DAF
		D138G	D138G	D138G	D138G		D138G
		D138N					D138N
		K166N
		K166E
	VP3	N63D	N63D	N63D	N63D			DAF
			N63H		N63H		N63H
		N63H	N63S
		N63Y	N63Y	N63Y
		Q234K	Q234K	Q234K	Q234K	Q234K	Q234K
			Q234L			Q234L
			Q234R		Q234R		Q234R
		E76G	E76G	E76G	E76G	E76G	E76G	unknown

The amino acid changes for each variant are listed according to capsid protein (VP1, VP2 or VP3), and their involvement in interacting with either or both receptors is indicated (receptor footprint).