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Published online 2016 Mar 18. doi: 10.1186/s12866-016-0671-1
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Table 4

PCR primers used in C. trachomatis conventional PCR and real-time PCR diagnosis. For nested PCR amplification of Momp and plasmid, outer primer pairs were used at first round PCR, while inner primer pairs were used at second round PCR amplification. For real-time PCR diagnosis, Momp inner primer pairs, plasmid Pgp8 inner primer pairs and plasmid Pgp1 primer pairs were used

Target genesPrimerSequence (5′-3′)Amplicon size
Momp Outer forwardTTGTTTTCGACCGTGTTTTG455 bp
Outer reverseAGCRTATTGGAAAGAAGCBCCTAA
Inner forwardAAACWGATGTGAATAAAGARTT395 bp
Inner reverseTCCCASARAGCTGCDCGAGC
Plasmid Pgp8 Outer forwardTTGGCYGCTAGAAAAGGCGATT212 bp
Outer reverseTCCGGAACAYATGATGCGAAGT
Inner forwardAACCAAGGTCGATGTGATAG150 bp
Inner reverseTCAGATAATTGGCGATTCTT
Plasmid Pgp1 ForwardTTCTTTGATGGCTTCCCAAC456 bp
ReverseACGATTTTCTCCAACCGATG
β-globin ForwardGAAGAGCCAAGGACAGGTAC268 bp
ReverseCAACTTCATCCACGTTCACC