Figure 1

(a) Representation of interactions between the natural product community, GNPS spectral libraries, and GNPS datasets. At present 221,083 MS/MS spectra from 18,163 unique compounds are used for the search in the GNPS. These include both 3^rd party libraries such as MassBank, ReSpect, and NIST, as well as spectral libraries created for GNPS (GNPS-Collections) and spectra from the natural product community (GNPS-Community). GNPS spectral libraries grow through user contributions of new identifications of MS/MS spectra. To date, 55 community members have contributed 8,853 MS/MS spectra from 5,568 unique compounds (30.5% of the unique compounds available). In addition, on-going curation efforts have already yielded 563 annotation updates for library spectra. The utility of these libraries is to dereplicate compounds (recognition previously characterized and studied known compounds), in both public and private data. This dereplication process is performed on all public datasets and results are automatically reported, thus enabling users to query all datasets/organisms/conditions. Automatic reanalysis of all public data creates a virtuous cycle in which contributions to libraries can be matched to all public data. Combined with molecular networking (Fig. 3), this automatic reanalysis empowers community members to identify analogs that can then be added to GNPS spectral libraries. (b) The GNPS platform has grown to serve a global user base of 9,200+ users from 100 countries.