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Abstract 


In ionic conditions in which clathrin coats are stable, the mannose-6-phosphate receptor associates with the 100-kd/50-kd coat complexes purified from bullock brain coated vesicles. These aggregates exist as striking spherical structures of 300-1000 A diameter. When clathrin is included in the assembly mixture, cages are formed which apparently encapsulate these aggregates, giving, in the absence of lipid, structures resembling full coated vesicles.

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EMBO J. 1985 Oct; 4(10): 2457–2460.
PMCID: PMC554529
PMID: 2865131

Assembly of the mannose-6-phosphate receptor into reconstituted clathrin coats.

Abstract

In ionic conditions in which clathrin coats are stable, the mannose-6-phosphate receptor associates with the 100-kd/50-kd coat complexes purified from bullock brain coated vesicles. These aggregates exist as striking spherical structures of 300-1000 A diameter. When clathrin is included in the assembly mixture, cages are formed which apparently encapsulate these aggregates, giving, in the absence of lipid, structures resembling full coated vesicles.

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Selected References

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  • Anderson RG, Goldstein JL, Brown MS. A mutation that impairs the ability of lipoprotein receptors to localise in coated pits on the cell surface of human fibroblasts. Nature. 1977 Dec 22;270(5639):695–699. [Abstract] [Google Scholar]
  • Bleil JD, Bretscher MS. Transferrin receptor and its recycling in HeLa cells. EMBO J. 1982;1(3):351–355. [Europe PMC free article] [Abstract] [Google Scholar]
  • Bretscher MS, Thomson JN, Pearse BM. Coated pits act as molecular filters. Proc Natl Acad Sci U S A. 1980 Jul;77(7):4156–4159. [Europe PMC free article] [Abstract] [Google Scholar]
  • Brown WJ, Farquhar MG. The mannose-6-phosphate receptor for lysosomal enzymes is concentrated in cis Golgi cisternae. Cell. 1984 Feb;36(2):295–307. [Abstract] [Google Scholar]
  • Brown WJ, Farquhar MG. Accumulation of coated vesicles bearing mannose 6-phosphate receptors for lysosomal enzymes in the Golgi region of I-cell fibroblasts. Proc Natl Acad Sci U S A. 1984 Aug;81(16):5135–5139. [Europe PMC free article] [Abstract] [Google Scholar]
  • Crowther RA, Pearse BM. Assembly and packing of clathrin into coats. J Cell Biol. 1981 Dec;91(3 Pt 1):790–797. [Europe PMC free article] [Abstract] [Google Scholar]
  • Goldstein JL, Anderson RG, Brown MS. Coated pits, coated vesicles, and receptor-mediated endocytosis. Nature. 1979 Jun 21;279(5715):679–685. [Abstract] [Google Scholar]
  • Karin M, Mintz B. Receptor-mediated endocytosis of transferrin in developmentally totipotent mouse teratocarcinoma stem cells. J Biol Chem. 1981 Apr 10;256(7):3245–3252. [Abstract] [Google Scholar]
  • Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. [Abstract] [Google Scholar]
  • Larrick JW, Cresswell P. Modulation of cell surface iron transferrin receptors by cellular density and state of activation. J Supramol Struct. 1979;11(4):579–586. [Abstract] [Google Scholar]
  • Pearse BM. Coated vesicles from human placenta carry ferritin, transferrin, and immunoglobulin G. Proc Natl Acad Sci U S A. 1982 Jan;79(2):451–455. [Europe PMC free article] [Abstract] [Google Scholar]
  • Pearse BM, Bretscher MS. Membrane recycling by coated vesicles. Annu Rev Biochem. 1981;50:85–101. [Abstract] [Google Scholar]
  • Pearse BM, Robinson MS. Purification and properties of 100-kd proteins from coated vesicles and their reconstitution with clathrin. EMBO J. 1984 Sep;3(9):1951–1957. [Europe PMC free article] [Abstract] [Google Scholar]
  • Perry MM, Gilbert AB. Yolk transport in the ovarian follicle of the hen (Gallus domesticus): lipoprotein-like particles at the periphery of the oocyte in the rapid growth phase. J Cell Sci. 1979 Oct;39:257–272. [Abstract] [Google Scholar]
  • Sahagian GG, Distler J, Jourdian GW. Characterization of a membrane-associated receptor from bovine liver that binds phosphomannosyl residues of bovine testicular beta-galactosidase. Proc Natl Acad Sci U S A. 1981 Jul;78(7):4289–4293. [Europe PMC free article] [Abstract] [Google Scholar]
  • Sly WS, Fischer HD. The phosphomannosyl recognition system for intracellular and intercellular transport of lysosomal enzymes. J Cell Biochem. 1982;18(1):67–85. [Abstract] [Google Scholar]
  • Tejedor F, Ballesta JP. Iodination of biological samples without loss of functional activity. Anal Biochem. 1982 Nov 15;127(1):143–149. [Abstract] [Google Scholar]

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