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The molecular basis of the specific anti-influenza action of amantadine.

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The EMBO Journal, 01 Nov 1985, 4(11):3021-3024
https://doi.org/10.1002/j.1460-2075.1985.tb04038.x PMID: 4065098 PMCID: PMC554613

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Abstract 

Amantadine (1-aminoadamantane hydrochloride) is effective in the prophylaxis and treatment of influenza A infections. In tissue culture this selective, strain-specific antiviral activity occurs at relatively low concentrations (5 microM or less), which inhibit either the initiation of infection or virus assembly. The data reported here demonstrate that the basis of these actions is similar and resides in the virus-coded M2 membrane protein, the product of a spliced transcript of RNA segment 7. Mutations which confer resistance to amantadine are restricted to four amino acids within a hydrophobic sequence, indicating that the drug is targetted against the putative membrane-associated portion of the molecule. The influence of the virus haemagglutinin on the amantadine sensitivity of virus strains implies that the drug may interfere with interactions between these two virus proteins.

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EMBO J. 1985 Nov; 4(11): 3021–3024.
PMCID: PMC554613
PMID: 4065098

The molecular basis of the specific anti-influenza action of amantadine.

A J Hay, A J Wolstenholme, J J Skehel, and M H Smith
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Abstract

Amantadine (1-aminoadamantane hydrochloride) is effective in the prophylaxis and treatment of influenza A infections. In tissue culture this selective, strain-specific antiviral activity occurs at relatively low concentrations (5 microM or less), which inhibit either the initiation of infection or virus assembly. The data reported here demonstrate that the basis of these actions is similar and resides in the virus-coded M2 membrane protein, the product of a spliced transcript of RNA segment 7. Mutations which confer resistance to amantadine are restricted to four amino acids within a hydrophobic sequence, indicating that the drug is targetted against the putative membrane-associated portion of the molecule. The influence of the virus haemagglutinin on the amantadine sensitivity of virus strains implies that the drug may interfere with interactions between these two virus proteins.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.
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