Europe PMC

This website requires cookies, and the limited processing of your personal data in order to function. By using the site you are agreeing to this as outlined in our privacy notice and cookie policy.

The beta2-microglobulin mRNA in human Daudi cells has a mutated initiation codon but is still inducible by interferon.

Author information

Affiliations

  • 1. Institut Pasteur, Université Paris VII, France.
      Authors
    • Rosa F 1
    (1 author)

ORCIDs linked to this article

The EMBO Journal, 01 Jan 1983, 2(2):239-243
https://doi.org/10.1002/j.1460-2075.1983.tb01412.x PMID: 11894933 PMCID: PMC555120

Free full text in Europe PMC

Abstract 

The human Burkitt lymphoma cell line Daudi does not synthesize beta2-microglobulin (beta2m) and lacks the cell surface histocompatibility antigens. The cells, however, contain RNA hybridizing to a cloned human beta2m cDNA probe. cDNA from this Daudi beta2m RNA, was cloned and sequenced. By comparison with cDNA prepared from Ramos cells, which synthesized microglobulin, we determined the sequence of the 20 amino acid long leader peptide of pre-beta2m and show that in Daudi cells the initiator ATG has been mutated to ATC. Although Daudi beta2m RNA cannot be translated, interferon induces the beta2m RNA in Daudi cells as well as in normal human cells.

Free full text 

Logo of embojLink to Publisher's site
EMBO J. 1983; 2(2): 239–243.
PMCID: PMC555120
PMID: 11894933

The beta2-microglobulin mRNA in human Daudi cells has a mutated initiation codon but is still inducible by interferon.

F Rosa, H Berissi, J Weissenbach, L Maroteaux, M Fellous, and M Revel
Author information Copyright and License information Disclaimer

Abstract

The human Burkitt lymphoma cell line Daudi does not synthesize beta2-microglobulin (beta2m) and lacks the cell surface histocompatibility antigens. The cells, however, contain RNA hybridizing to a cloned human beta2m cDNA probe. cDNA from this Daudi beta2m RNA, was cloned and sequenced. By comparison with cDNA prepared from Ramos cells, which synthesized microglobulin, we determined the sequence of the 20 amino acid long leader peptide of pre-beta2m and show that in Daudi cells the initiator ATG has been mutated to ATC. Although Daudi beta2m RNA cannot be translated, interferon induces the beta2m RNA in Daudi cells as well as in normal human cells.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (1024K), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.
 
icon of scanned page 239
239
icon of scanned page 240
240
icon of scanned page 241
241
icon of scanned page 242
242
icon of scanned page 243
243
 

Images in this article

Fig. 1.
Fig. 1.
on p.240
Click on the image to see a larger version.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Adams A, Strander H, Cantell K. Sensitivity of the Epstein-Barr virus transformed human lymphoid cell lines to interferon. J Gen Virol. 1975 Aug;28(2):207–217. [Abstract] [Google Scholar]
  • Aleström P, Akusjärvi G, Perricaudet M, Mathews MB, Klessig DF, Pettersson U. The gene for polypeptide IX of adenovirus type 2 and its unspliced messenger RNA. Cell. 1980 Mar;19(3):671–681. [Abstract] [Google Scholar]
  • Arce-Gomez B, Jones EA, Barnstable CJ, Solomon E, Bodmer WF. The genetic control of HLA-A and B antigens in somatic cell hybrids: requirement for beta2 microglobulin. Tissue Antigens. 1978 Feb;11(2):96–112. [Abstract] [Google Scholar]
  • Auffray C, Rougeon F. Purification of mouse immunoglobulin heavy-chain messenger RNAs from total myeloma tumor RNA. Eur J Biochem. 1980 Jun;107(2):303–314. [Abstract] [Google Scholar]
  • Cunningham BA, Wang JL, Berggård I, Peterson PA. The complete amino acid sequence of beta 2-microglobulin. Biochemistry. 1973 Nov 20;12(24):4811–4822. [Abstract] [Google Scholar]
  • Denhardt DT. A membrane-filter technique for the detection of complementary DNA. Biochem Biophys Res Commun. 1966 Jun 13;23(5):641–646. [Abstract] [Google Scholar]
  • Fellous M, Bono R, Hyafil F, Gresser I. Interferon enhances the amount of membrane-bound beta2-microglobulin and its release from human Burkitt cells. Eur J Immunol. 1981 Jun;11(6):524–526. [Abstract] [Google Scholar]
  • Fellous M, Nir U, Wallach D, Merlin G, Rubinstein M, Revel M. Interferon-dependent induction of mRNA for the major histocompatibility antigens in human fibroblasts and lymphoblastoid cells. Proc Natl Acad Sci U S A. 1982 May;79(10):3082–3086. [Europe PMC free article] [Abstract] [Google Scholar]
  • Gates FT, 3rd, Coligan JE, Kindt TJ. Complete amino acid sequence of murine beta 2-microglobulin: structural evidence for strain-related polymorphism. Proc Natl Acad Sci U S A. 1981 Jan;78(1):554–558. [Europe PMC free article] [Abstract] [Google Scholar]
  • Grunstein M, Hogness DS. Colony hybridization: a method for the isolation of cloned DNAs that contain a specific gene. Proc Natl Acad Sci U S A. 1975 Oct;72(10):3961–3965. [Europe PMC free article] [Abstract] [Google Scholar]
  • Guerry P, LeBlanc DJ, Falkow S. General method for the isolation of plasmid deoxyribonucleic acid. J Bacteriol. 1973 Nov;116(2):1064–1066. [Europe PMC free article] [Abstract] [Google Scholar]
  • Klein E, Klein G, Nadkarni JS, Nadkarni JJ, Wigzell H, Clifford P. Surface IgM-kappa specificity on a Burkitt lymphoma cell in vivo and in derived culture lines. Cancer Res. 1968 Jul;28(7):1300–1310. [Abstract] [Google Scholar]
  • Klein G, Zeuthen J, Terasaki P, Billing R, Honig R, Jondal M, Westman A, Clements G. Inducibility of the Epstein-Barr virus (EBV) cycle and surface marker properties of EBV-negative lymphoma lines and their in vitro EBV-converted sublines. Int J Cancer. 1976 Nov 15;18(5):639–652. [Abstract] [Google Scholar]
  • Kozak M. How do eucaryotic ribosomes select initiation regions in messenger RNA? Cell. 1978 Dec;15(4):1109–1123. [Abstract] [Google Scholar]
  • LeMeur M, Glanville N, Mandel JL, Gerlinger P, Palmiter R, Chambon P. The ovalbumin gene family: hormonal control of X and Y gene transcription and mRNA accumulation. Cell. 1981 Feb;23(2):561–571. [Abstract] [Google Scholar]
  • Lomedico PT, McAndrew SJ. Eukaryotic ribosomes can recognize preproinsulin initiation codons irrespective of their position relative to the 5' end of mRNA. Nature. 1982 Sep 16;299(5880):221–226. [Abstract] [Google Scholar]
  • Lingappa VR, Cunningham BA, Jazwinski SM, Hopp TP, Blobel G, Edelman GM. Cell-free synthesis and segregation of beta 2-microglobulin. Proc Natl Acad Sci U S A. 1979 Aug;76(8):3651–3655. [Europe PMC free article] [Abstract] [Google Scholar]
  • Maxam AM, Gilbert W. Sequencing end-labeled DNA with base-specific chemical cleavages. Methods Enzymol. 1980;65(1):499–560. [Abstract] [Google Scholar]
  • Myers JC, Spiegelman S. Sodium pyrophosphate inhibition of RNA.DNA hybrid degradation by reverse transcriptase. Proc Natl Acad Sci U S A. 1978 Nov;75(11):5329–5333. [Europe PMC free article] [Abstract] [Google Scholar]
  • Nilsson K, Evrin PE, Welsh KI. Production of beta 2-microglobulin by normal and malignant human cell lines and peripheral lymphocytes. Transplant Rev. 1974;21(0):53–84. [Abstract] [Google Scholar]
  • Parnes JR, Seidman JG. Structure of wild-type and mutant mouse beta 2-microglobulin genes. Cell. 1982 Jun;29(2):661–669. [Abstract] [Google Scholar]
  • Ploegh HL, Cannon LE, Strominger JL. Cell-free translation of the mRNAs for the heavy and light chains of HLA-A and HLA-B antigens. Proc Natl Acad Sci U S A. 1979 May;76(5):2273–2277. [Europe PMC free article] [Abstract] [Google Scholar]
  • Rigby PW, Dieckmann M, Rhodes C, Berg P. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. J Mol Biol. 1977 Jun 15;113(1):237–251. [Abstract] [Google Scholar]
  • Roskam WG, Rougeon F. Molecular cloning and nucleotide sequence of the human growth hormone structural gene. Nucleic Acids Res. 1979 Sep 25;7(2):305–320. [Europe PMC free article] [Abstract] [Google Scholar]
  • Rougeon F, Mach B. Cloning and amplification of rabbit alpha- and beta-globin gene sequences into Escherichia coli plasmids. J Biol Chem. 1977 Apr 10;252(7):2209–2217. [Abstract] [Google Scholar]
  • Rubinstein M, Levy WP, Moschera JA, Lai CY, Hershberg RD, Bartlett RT, Pestka S. Human leukocyte interferon: isolation and characterization of several molecular forms. Arch Biochem Biophys. 1981 Aug;210(1):307–318. [Abstract] [Google Scholar]
  • Shulman L, Revel M. Interferon-dependent induction of mRNA activity for (2'-5')oligo-isoadenylate synthetase. Nature. 1980 Nov 6;288(5786):98–100. [Abstract] [Google Scholar]
  • Suggs SV, Wallace RB, Hirose T, Kawashima EH, Itakura K. Use of synthetic oligonucleotides as hybridization probes: isolation of cloned cDNA sequences for human beta 2-microglobulin. Proc Natl Acad Sci U S A. 1981 Nov;78(11):6613–6617. [Europe PMC free article] [Abstract] [Google Scholar]
  • Thomas PS. Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose. Proc Natl Acad Sci U S A. 1980 Sep;77(9):5201–5205. [Europe PMC free article] [Abstract] [Google Scholar]
  • Wallach D, Fellous M, Revel M. Preferential effect of gamma interferon on the synthesis of HLA antigens and their mRNAs in human cells. Nature. 1982 Oct 28;299(5886):833–836. [Abstract] [Google Scholar]
  • Weatherall DJ, Clegg JB. Molecular genetics of human hemoglobin. Annu Rev Genet. 1976;10:157–178. [Abstract] [Google Scholar]
  • Ziegler A, Laudien D, Heinrichs H, Müller C, Uchańska-Ziegler B, Wernet P. K562 cells express human major histocompatibility antigens. Immunogenetics. 1981;13(4):359–365. [Abstract] [Google Scholar]
Articles from The EMBO Journal are provided here courtesy of Nature Publishing Group

Full text links 

Read article at publisher's site: https://doi.org/10.1002/j.1460-2075.1983.tb01412.x

Read article for free, from open access legal sources, via Unpaywall: https://onlinelibrary.wiley.com/doi/pdfdirect/10.1002/j.1460-2075.1983.tb01412.xUnpaywall

Citations & impact 

Impact metrics

80
Citations
Jump to Citations
5
Data citations
Jump to Data

Citations of article over time

Alternative metrics

Altmetric item for https://www.altmetric.com/details/22672006
Altmetric
Discover the attention surrounding your research
https://www.altmetric.com/details/22672006

Smart citations by scite.ai
Smart citations by scite.ai include citation statements extracted from the full text of the citing article. The number of the statements may be higher than the number of citations provided by EuropePMC if one paper cites another multiple times or lower if scite has not yet processed some of the citing articles.
Explore citation contexts and check if this article has been supported or disputed.
https://scite.ai/reports/10.1002/j.1460-2075.1983.tb01412.x

Supporting
Mentioning
Contrasting
2
74
0

Article citations

Go to all (80) article citations

Data 

Protocols & materials 

Similar Articles 

To arrive at the top five similar articles we use a word-weighted algorithm to compare words from the Title and Abstract of each citation.