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Published online 2020 Jan 13. doi: 10.1038/s41598-019-56867-0

Figure 2

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AP-MS analysis of Hho1-FZZ in three different physiological states of the Tetrahymena life cycle. Top: Western blotting analysis indicating recovery of the affinity purified Hho1-FZZ bait protein. The top panel represents the vegetative samples and the bottom panel shows the recovery of the bait from conjugation samples. The blots were probed with anti-FLAG antibody. No signal was detected in samples from the untagged control cells. Bottom: Network illustration of the Hho1-FZZ co-purifying proteins at statistical threshold of FDR  1% in three different stages of the Tetrahymena life cycle; vegetative growth, starvation (24 hours) and conjugation (6 hours post mixing of the cells). Node color key is provided.

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