Figure 6

Focused Activin + TGF-β subfamily prodomain alignment indicating structural conservation. Sequences presented as in Figure 2 except gaps are omitted for brevity and indicated by columns of black dots. Numbering is only approximate. Locations and naming of structural features are from TGF-β1 (Shi et al. 2011). Underlined α2 sequences of TGF-β1 and Inhibin-βa indicate distinct locations. Underlined β1 in TGF-β1 shows it is in a distinct location in Inhibin-βa. Underline of α3 in TGF-β1 indicates absence of conservation. The notation TGF-β with β3 indicates this feature approximates the TGF-β1 pattern (underlined). The notation Activin with β7 indicates this feature approximates the Activin pattern (underlined) not the underlined TGF-β1 pattern. In β8 of the bowtie of TGF-β1–3, the conserved pair of cysteines (CxC) is underlined in black. In β8 Maverick and Dawdle are joined by Activin, Inhibin-βa, and Inhibin-βb, with a conserved pair of cysteines of distinct spacing (red underline; CxxC). Underlined β9, β9′, and α5 are not conserved.