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FIG. 4

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PCR analysis of vesicle DNA for the presence of gfp. Vesicles isolated from E. coli O157:H7 harboring pGFP were treated with DNase and used as a template for PCR analysis with gfp primers. Left lane, vesicles containing gfp; right lane, purified pGFP (control). The molecular size of the expected fragment was 800 bp.

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