Phenotyping for QTL identification: A case study of resistance to Plasmopara viticola and Erysiphe necator in grapevine

doi:10.3389/fpls.2022.930954

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Front Plant Sci. 2022; 13: 930954.

Published online 2022 Aug 11. doi: 10.3389/fpls.2022.930954

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Table 3

Resistance variables assessed in the vineyard and greenhouse. An abbreviation and a description of the method and scale utilized for the scoring is proposed for each resistance variable assessed in mapping studies.

Resistance variable ID	Trait	Method	Scale (susceptible to resistant)
OIV 452; OIV 452i	Leaf degree of resistance to P. viticola	Visual semi-quantitative estimation of pathogen infection	Discrete 1 to 9; Discrete 9 to 1 (i)
OIV 453; OIV 453i	Cluster degree of resistance to P. viticola	Visual semi-quantitative estimation of pathogen infection	Discrete 1 to 9; Discrete 9 to 1 (i)
DOS	Leaf degree of resistance to P. viticola	Visual measurement of the diameter of oil spots (DOS)	Centimeters
OIV 455; OIV 455i (or IPGRI)	Leaf degree of resistance to E. necator	Visual semi-quantitative estimation of pathogen infection	Discrete 1 to 9; Discrete 9 to 1 (i)
OIV 456; OIV 456i	Cluster degree of resistance to E. necator	Visual semi-quantitative estimation of pathogen infection	Discrete 1 to 9; Discrete 9 to 1 (i)
DI	Leaf degree of resistance to P. viticola or E. necator	Disease incidence (DI): visual count or classification of infected plants/organs	Number of counted units
DS	Leaf degree of resistance to P. viticola or E. necator	Disease severity (DS): visual semi-quantitative estimation of the infection	Discrete: 1 to 5 (Dalbó, 1998); 1 to 4 (Ramming et al., 2011; Karn et al., 2021); 0 to 5 (Riaz et al., 2011; Pap et al., 2016); 1 to 7 (Teh et al., 2017); 1 to 9 (Karn et al., 2021); 1 to 10 (Bhattarai et al., 2021)
NS	Leaf degree of resistance to P. viticola	Necrosis severity/size (NS): visual semi-quantitative estimation	Discrete 9 to 1
AUDPC	Leaf degree of resistance to P. viticola or E. necator	Calculation according to Jeger and Viljanen-Rollinson (2001)	According to the scale of single time point assessment

Abbreviations: AUDPC, Area Under Disease Pressure Curve; i, inverted resistance scale; ID, identity; IPGRI, International Plant Genetic Resources Institute; OIV, Organisation Internationale de la Vigne et du Vin.