1,2,3,4,6-Penta-O-galloyl-beta-D-glucose protects rat neuronal cells (Neuro 2A) from hydrogen peroxide-mediated cell death via the induction of heme oxygenase-1

Byung-Min Choi; Hyung-Jin Kim; Gi-Su Oh; Hyun-Ock Pae; Hyuncheol Oh; Sejin Jeong; Tae-Oh Kwon; Young-Myeong Kim; Hun-Taeg Chung

doi:10.1016/s0304-3940(02)00513-x

1,2,3,4,6-Penta-O-galloyl-beta-D-glucose protects rat neuronal cells (Neuro 2A) from hydrogen peroxide-mediated cell death via the induction of heme oxygenase-1

Neurosci Lett. 2002 Aug 9;328(2):185-9. doi: 10.1016/s0304-3940(02)00513-x.

Authors

Byung-Min Choi¹, Hyung-Jin Kim, Gi-Su Oh, Hyun-Ock Pae, Hyuncheol Oh, Sejin Jeong, Tae-Oh Kwon, Young-Myeong Kim, Hun-Taeg Chung

Affiliation

¹ Medicinal Resources Research Center (MRRC), Wonkwang University, Iksan, 570-749, Chonbuk, South Korea.

PMID: 12133584
DOI: 10.1016/s0304-3940(02)00513-x

Abstract

The root of Paeonia suffruticosa ANDREWS is an important Chinese crude drug used in many traditional prescriptions. 1,2,3,4,6-Penta-O-galloyl-beta-D-glucose (PGG), a major component of this crude drug, has been shown to possess potent anti-oxidant, anti-mutagenic and anti-proliferative effects. In the present study, we examined the effect of PGG on the expression of neuronal heme oxygenase-1 (HO-1), an inducible stress protein that degrades heme to the neuroactive molecule, carbon monoxide and the anti-oxidant, biliverdin. Exposure of Neuro 2A cells to PGG (10-50 microM) resulted in a concentration- and time-dependent induction of HO-1 mRNA, and protein expressions and heme oxygenase activity. Interestingly, pretreatment of the neuronal cells with PGG resulted in enhanced cellular resistance to hydrogen peroxide. This cytoprotective effect was reversed by zinc protoporphyrin IX, an inhibitor of heme oxygenase. This study showed that PGG could protect neuronal cells from oxidative stress via the induction of HO-1 gene expression.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Antioxidants / pharmacology*
Brain Ischemia / drug therapy
Brain Ischemia / enzymology
Brain Ischemia / physiopathology
Cell Death / drug effects*
Cell Death / physiology
Dose-Response Relationship, Drug
Drugs, Chinese Herbal / pharmacology
Gene Expression Regulation, Enzymologic / drug effects
Gene Expression Regulation, Enzymologic / physiology
Heme Oxygenase (Decyclizing) / drug effects*
Heme Oxygenase (Decyclizing) / genetics
Heme Oxygenase (Decyclizing) / metabolism
Heme Oxygenase-1
Hydrogen Peroxide / antagonists & inhibitors*
Hydrogen Peroxide / metabolism
Hydrolyzable Tannins*
Neuroblastoma
Neurodegenerative Diseases / drug therapy
Neurodegenerative Diseases / enzymology
Neurodegenerative Diseases / physiopathology
Neurons / drug effects*
Neurons / enzymology
Neuroprotective Agents / pharmacology
Oxidative Stress / drug effects*
Oxidative Stress / physiology
RNA, Messenger / drug effects
RNA, Messenger / metabolism
Rats
Tannins / pharmacology*
Tumor Cells, Cultured

Substances

Antioxidants
Drugs, Chinese Herbal
Hydrolyzable Tannins
Neuroprotective Agents
RNA, Messenger
Tannins
pentagalloylglucose
Hydrogen Peroxide
Heme Oxygenase (Decyclizing)
Heme Oxygenase-1