Abstract
Human peripheral blood NK cells may be divided into two main subsets: CD56(bright)CD16(-) and CD56(dim)CD16(+). Since TGF-β is known to influence the development of many leukocyte lineages, its effects on NK cell differentiation either from human CD34(+)Lin(-) hematopoietic progenitor/stem cells in vitro or from peripheral blood NK cells were investigated. TGF-β represses development of NK cells from CD34(+) progenitors and inhibits differentiation of CD16(+) NK cells. Moreover, TGF-β also results in conversion of a minor fraction of CD56(bright)CD16(+) cells found in peripheral blood into CD56(bright)CD16(-) cells, highlighting a possible role of the former as a developmental intermediate and of TGF-β in influencing the genesis of NK subsets found in blood.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Antibodies, Blocking / pharmacology
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Antigens, CD / biosynthesis
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Antigens, CD / genetics
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Antigens, Differentiation / biosynthesis
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Antigens, Differentiation / genetics
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Bone Marrow / pathology
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Cell Differentiation* / drug effects
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Cell Differentiation* / immunology
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Cell Lineage / drug effects
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Cell Lineage / immunology
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Cells, Cultured
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Cytokines / metabolism
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Fetal Blood / cytology
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Hematopoietic Stem Cells / drug effects
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Hematopoietic Stem Cells / metabolism*
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Hematopoietic Stem Cells / pathology
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Humans
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Killer Cells, Natural / drug effects
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Killer Cells, Natural / immunology
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Killer Cells, Natural / metabolism*
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Killer Cells, Natural / pathology
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Lymphocyte Subsets / drug effects
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Lymphocyte Subsets / immunology
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Lymphocyte Subsets / metabolism*
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Lymphocyte Subsets / pathology
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Transforming Growth Factor beta / immunology
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Transforming Growth Factor beta / pharmacology*
Substances
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Antibodies, Blocking
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Antigens, CD
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Antigens, Differentiation
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Cytokines
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Transforming Growth Factor beta