Live simian immunodeficiency virus vaccine correlate of protection: local antibody production and concentration on the path of virus entry

J Immunol. 2014 Sep 15;193(6):3113-25. doi: 10.4049/jimmunol.1400820. Epub 2014 Aug 18.

Abstract

We sought design principles for a vaccine to prevent HIV transmission to women by identifying correlates of protection conferred by a highly effective live attenuated SIV vaccine in the rhesus macaque animal model. We show that SIVmac239Δnef vaccination recruits plasma cells and induces ectopic lymphoid follicle formation beneath the mucosal epithelium in the rhesus macaque female reproductive tract. The plasma cells and ectopic follicles produce IgG Abs reactive with viral envelope glycoprotein gp41 trimers, and these Abs are concentrated on the path of virus entry by the neonatal FcR in cervical reserve epithelium and in vaginal epithelium. This local Ab production and delivery system correlated spatially and temporally with the maturation of local protection against high-dose pathogenic SIV vaginal challenge. Thus, designing vaccines to elicit production and concentration of Abs at mucosal frontlines could aid in the development of an effective vaccine to protect women against HIV-1.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Neutralizing / immunology
  • Antibodies, Viral / immunology
  • Antibody Formation / immunology
  • Cervix Uteri / immunology*
  • Cervix Uteri / virology
  • Female
  • HIV Envelope Protein gp41 / immunology
  • HIV Infections / immunology
  • HIV Infections / prevention & control
  • HIV-1 / immunology
  • Immunoglobulin G / biosynthesis
  • Immunoglobulin G / immunology
  • Macaca mulatta
  • Mucous Membrane / immunology
  • SAIDS Vaccines / administration & dosage
  • SAIDS Vaccines / immunology*
  • Simian Acquired Immunodeficiency Syndrome / immunology
  • Simian Acquired Immunodeficiency Syndrome / prevention & control*
  • Simian Immunodeficiency Virus / immunology*
  • Vaccination
  • Vaccines, Attenuated / administration & dosage
  • Vaccines, Attenuated / immunology
  • Vagina / immunology*
  • Vagina / virology

Substances

  • Antibodies, Neutralizing
  • Antibodies, Viral
  • HIV Envelope Protein gp41
  • Immunoglobulin G
  • SAIDS Vaccines
  • Vaccines, Attenuated